Literature summary for 5.1.99.5 extracted from

Martinez-Rodriguez, S.; Las Heras-Vazquez, F.J.; Mingorance-Cazorla, L.; Clemente-Jimenez, J.M.; Rodriguez-Vico, F.
Molecular cloning, purification, and biochemical characterization of hydantoin racemase from the legume symbiont Sinorhizobium meliloti CECT 4114 (2004), Appl. Environ. Microbiol., 70, 625-630.

Search for more literature for 5.1.99.5

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Sinorhizobium meliloti

General Stability

General Stability	Organism
the enzyme remains active after 10 freeze-thawing cycles	Sinorhizobium meliloti

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.76	-	D-isobutylhydantoin	pH 8.5	Sinorhizobium meliloti
6.41	-	L-isobutylhydantoin	pH 8.5	Sinorhizobium meliloti
8.3	-	L-benzylhydantoin	pH 8.5	Sinorhizobium meliloti
13.89	-	D-benzylhydantoin	pH 8.5	Sinorhizobium meliloti
17.32	-	L-ethylhydantoin	pH 8.5	Sinorhizobium meliloti

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
31000	-	4 * 31000, SDS-PAGE	Sinorhizobium meliloti
100000	-	gel filtration	Sinorhizobium meliloti

Organism

Organism	UniProt	Comment	Textmining
Sinorhizobium meliloti	-	-	-
Sinorhizobium meliloti CECT 4114	-	-	-

Storage Stability

Storage Stability	Organism
-20°C, 0.1 M Tris, 20% glycerol, pH 8.5, stable for at least 3 months	Sinorhizobium meliloti
4°C, 0.1 M Tris, pH 8.5, stable for 10 weeks	Sinorhizobium meliloti

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-benzylhydantoin	-	Sinorhizobium meliloti	L-benzylhydantoin	-	r
D-benzylhydantoin	-	Sinorhizobium meliloti CECT 4114	L-benzylhydantoin	-	r
D-isobutylhydantoin	-	Sinorhizobium meliloti	L-isobutylhydantoin	-	r
D-isobutylhydantoin	-	Sinorhizobium meliloti CECT 4114	L-isobutylhydantoin	-	r
L-benzylhydantoin	-	Sinorhizobium meliloti	D-benzylhydantoin	-	r
L-benzylhydantoin	-	Sinorhizobium meliloti CECT 4114	D-benzylhydantoin	-	r
L-ethylhydantoin	-	Sinorhizobium meliloti	D-ethylhydantoin	-	r
L-ethylhydantoin	-	Sinorhizobium meliloti CECT 4114	D-ethylhydantoin	-	r
L-isobutylhydantoin	-	Sinorhizobium meliloti	D-isobutylhydantoin	-	r
L-isobutylhydantoin	-	Sinorhizobium meliloti CECT 4114	D-isobutylhydantoin	-	r

Subunits

Subunits	Comment	Organism
tetramer	4 * 31000, SDS-PAGE	Sinorhizobium meliloti

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	-	Sinorhizobium meliloti

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.94	-	L-benzylhydantoin	pH 8.5	Sinorhizobium meliloti
2.12	-	L-isobutylhydantoin	pH 8.5	Sinorhizobium meliloti
2.29	-	D-benzylhydantoin	pH 8.5	Sinorhizobium meliloti
3.24	-	D-isobutylhydantoin	pH 8.5	Sinorhizobium meliloti
6.42	-	L-ethylhydantoin	pH 8.5	Sinorhizobium meliloti

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8.5	-	-	Sinorhizobium meliloti

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)