Application | Comment | Organism |
---|---|---|
medicine | alpha-methylacyl-coenzyme A racemase is a biomarker for prostate cancer and a putative target for the development of therapeutic agents directed against the disease, inhibition of enzyme activity may also act synergistically with androgen ablation therapy | Mycobacterium tuberculosis |
Cloned (Comment) | Organism |
---|---|
the open reading frame encoding alpha-methylacyl-coenzyme A racemase from Mycobacterium tuberculosis is subcloned into a pET15b vector, and the enzyme is overexpressed in Escherichia coli cells | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(2R)-ibuprofenoyl-CoA | competitive inhibitor | Mycobacterium tuberculosis | |
(2S)-ibuprofenoyl-CoA | competitive inhibitor | Mycobacterium tuberculosis | |
octyl-beta-D-glucopyranoside | concentrations greater than 0.6% octyl-beta-D-glucopyranoside cause a reduction in enzyme activity | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.048 | - |
(2R)-ibuprofenoyl-CoA | recombinant enzyme, 50 mM Tris-HCl, 0.2% octyl-beta-D-glucopyranoside, pH 8.0 at 37°C | Mycobacterium tuberculosis | |
0.071 | - |
(2R)-ibuprofenoyl-CoA | recombinant six-His tagged enzyme, 50 mM Tris-HCl, 0.2% octyl-beta-D-glucopyranoside, pH 8.0 at 37°C | Mycobacterium tuberculosis | |
0.086 | - |
(2S)-ibuprofenoyl-CoA | recombinant enzyme, 50 mM Tris-HCl, 0.2% octyl-beta-D-glucopyranoside, pH 8.0 at 37°C | Mycobacterium tuberculosis | |
0.087 | - |
(2S)-ibuprofenoyl-CoA | recombinant six-His tagged enzyme, 50 mM Tris-HCl, 0.2% octyl-beta-D-glucopyranoside, pH 8.0 at 37°C | Mycobacterium tuberculosis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | - |
Mycobacterium tuberculosis | 5777 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38970 | - |
calculated for recombinant enzyme without six-His tag | Mycobacterium tuberculosis |
40850 | - |
calculated for recombinant enzyme with six-His tag | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by using metal ion affinity chromatography | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R)-ibuprofenoyl-CoA | - |
Mycobacterium tuberculosis | (2S)-ibuprofenoyl-CoA | - |
r | |
(2R)-pristanoyl-CoA | - |
Mycobacterium tuberculosis | (2S)-pristanoyl-CoA | - |
r | |
3alpha,7alpha,12alpha-trihydroxy-5beta-cholestanoyl-CoA | - |
Mycobacterium tuberculosis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
alpha-methylacyl-coenzyme A racemase | - |
Mycobacterium tuberculosis |
AMACR | - |
Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
228 | - |
(2R)-ibuprofenoyl-CoA | recombinant six-His tagged enzyme, 50 mM Tris-HCl, 0.2% octyl-beta-D-glucopyranoside, pH 8.0 at 37°C | Mycobacterium tuberculosis | |
291 | - |
(2R)-ibuprofenoyl-CoA | recombinant enzyme, 50 mM Tris-HCl, 0.2% octyl-beta-D-glucopyranoside, pH 8.0 at 37°C | Mycobacterium tuberculosis | |
358 | - |
(2S)-ibuprofenoyl-CoA | recombinant six-His tagged enzyme, 50 mM Tris-HCl, 0.2% octyl-beta-D-glucopyranoside, pH 8.0 at 37°C | Mycobacterium tuberculosis | |
450 | - |
(2S)-ibuprofenoyl-CoA | recombinant enzyme, 50 mM Tris-HCl, 0.2% octyl-beta-D-glucopyranoside, pH 8.0 at 37°C | Mycobacterium tuberculosis |
General Information | Comment | Organism |
---|---|---|
metabolism | beta-oxidation pathway of fatty acids | Mycobacterium tuberculosis |
physiological function | enzyme catalyzes the 1,1-proton transfer reaction that effects the reversible epimerization of the coenzyme A thioesters derived from a variety of (2R)- and (2S)-methyl branched fatty acids | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Mycobacterium tuberculosis enzyme expressed as a fusion protein bearing an N-terminal six-His tag has a catalytic efficiency that is reduced 22% and 47% in the 2S-2R and 2R-2S directions, respectively, relative to untagged enzyme | Mycobacterium tuberculosis | |
0.0032 | - |
(2R)-ibuprofenoyl-CoA | recombinant six-His tagged enzyme, 50 mM Tris-HCl, 0.2% octyl-beta-D-glucopyranoside, pH 8.0 at 37°C | Mycobacterium tuberculosis | |
0.0041 | - |
(2S)-ibuprofenoyl-CoA | recombinant six-His tagged enzyme, 50 mM Tris-HCl, 0.2% octyl-beta-D-glucopyranoside, pH 8.0 at 37°C | Mycobacterium tuberculosis | |
0.0052 | - |
(2S)-ibuprofenoyl-CoA | recombinant enzyme, 50 mM Tris-HCl, 0.2% octyl-beta-D-glucopyranoside, pH 8.0 at 37°C | Mycobacterium tuberculosis | |
0.0061 | - |
(2R)-ibuprofenoyl-CoA | recombinant enzyme, 50 mM Tris-HCl, 0.2% octyl-beta-D-glucopyranoside, pH 8.0 at 37°C | Mycobacterium tuberculosis |