Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | preincubation for 30 min at 30 C stimulates | Comamonas testosteroni |
General Stability | Organism |
---|---|
repeated freezing and thawing inactivates | Comamonas testosteroni |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Comamonas testosteroni | |
5-Aminohydantoin | - |
Comamonas testosteroni | |
Co2+ | 0.8 mM | Comamonas testosteroni | |
hydantoin | - |
Comamonas testosteroni | |
Hydantoin of N-carbamoyl-L-methionine | - |
Comamonas testosteroni | |
iodoacetate | - |
Comamonas testosteroni | |
Mn2+ | 0.8 mM | Comamonas testosteroni | |
NH2OH | - |
Comamonas testosteroni | |
Zn2+ | 0.8 mM | Comamonas testosteroni |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Comamonas testosteroni | - |
- |
- |
Pseudomonas fluorescens | - |
- |
- |
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Comamonas testosteroni |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Comamonas testosteroni |
Storage Stability | Organism |
---|---|
4°C, stable for several weeks | Comamonas testosteroni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)(+)-Allantoin | - |
Pseudomonas fluorescens | (R)(-)-Allantoin | - |
? | |
(S)(+)-Allantoin | - |
Pseudomonas putida | (R)(-)-Allantoin | - |
? | |
(S)(+)-Allantoin | r | Comamonas testosteroni | (R)(-)-Allantoin | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Comamonas testosteroni |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | 20°C: about 35% of maximal activity, 30°C: 67% of maximal activity, 60°C: about 85% of maximal activity | Comamonas testosteroni |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
15 min, pH 6.5-7, stable up to | Comamonas testosteroni |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.2 | - |
Comamonas testosteroni |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | up to 70°C, 15 min, stable | Comamonas testosteroni |