BRENDA - Enzyme Database
show all sequences of 5.1.99.1

Use of methylmalonyl-CoA epimerase in enhancing crotonase stereoselectivity

Hamed, R.B.; Gomez-Castellanos, J.R.; Sean Froese, D.; Krysztofinska, E.; Yue, W.W.; Schofield, C.J.; ChemBioChem 17, 471-473 (2016)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
recombinant methylmalonyl-CoA epimerase enables biocatalytic epimerisation of C-2 methyl- and ethylmalonyl-CoA. When coupled with variant carboxymethylproline synthase CarB and crotonyl-CoA carboxylase reductase, the methylmalonyl-CoA epimerase forms part of a three-enzyme, one-pot procedure for the production of functionalised C-5 carboxyalkylprolines from crotonyl-CoA, carbon dioxide and L-glutamate semialdehyde/5-hydroxyproline/pyrroline-5-carboxylate
Homo sapiens
Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Homo sapiens
5739
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
Q96PE7
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2R)-methylmalonyl-CoA
-
747542
Homo sapiens
(2S)-methylmalonyl-CoA
-
-
-
?
(2S)-ethylmalonyl-CoA
-
747542
Homo sapiens
(2R)-ethylmalonyl-CoA
-
-
-
?
Application (protein specific)
Application
Commentary
Organism
synthesis
recombinant methylmalonyl-CoA epimerase enables biocatalytic epimerisation of C-2 methyl- and ethylmalonyl-CoA. When coupled with variant carboxymethylproline synthase CarB and crotonyl-CoA carboxylase reductase, the methylmalonyl-CoA epimerase forms part of a three-enzyme, one-pot procedure for the production of functionalised C-5 carboxyalkylprolines from crotonyl-CoA, carbon dioxide and L-glutamate semialdehyde/5-hydroxyproline/pyrroline-5-carboxylate
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Homo sapiens
5739
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2R)-methylmalonyl-CoA
-
747542
Homo sapiens
(2S)-methylmalonyl-CoA
-
-
-
?
(2S)-ethylmalonyl-CoA
-
747542
Homo sapiens
(2R)-ethylmalonyl-CoA
-
-
-
?
Other publictions for EC 5.1.99.1
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748590
Gonzalez-Garcia
Awakening sleeping beauty Pro ...
Acidipropionibacterium acidipropionici, Acidipropionibacterium acidipropionici DSM 20272
Microb. Cell Fact.
16
121
2017
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747542
Hamed
Use of methylmalonyl-CoA epim ...
Homo sapiens
ChemBioChem
17
471-473
2016
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726729
Han
Epimerase (Msed_0639) and muta ...
Metallosphaera sedula, Metallosphaera sedula DSM 5348
Appl. Environ. Microbiol.
78
6194-6202
2012
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2
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1
1
1
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714552
Boghigian
Multi-factorial engineering of ...
Streptomyces coelicolor, Streptomyces coelicolor A3(2)
Biotechnol. Bioeng.
108
1360-1371
2011
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1
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46
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727331
Yang
-
Construction of an engineered ...
Streptomyces coelicolor
Chin. J. Antibiot.
36
191-196
2011
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1
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706660
Shi
Crystal structure of a putativ ...
Caldanaerobacter subterraneus subsp. tengcongensis
Proteins
77
994-999
2009
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1
1
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1
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1
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1
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693174
Erb
Ethylmalonyl-CoA mutase from R ...
Rhodobacter sphaeroides
J. Biol. Chem.
283
32283-32293
2008
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673023
Gross
Metabolic engineering of Pseud ...
Sorangium cellulosum
Chem. Biol.
13
1253-1264
2006
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1
1
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673947
Bikker
A homozygous nonsense mutation ...
Homo sapiens
Hum. Mutat.
27
640-643
2006
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1
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676026
Dobson
Homozygous nonsense mutation i ...
Homo sapiens
Mol. Genet. Metab.
88
327-333
2006
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1
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676032
Chandler
Propionyl-CoA and adenosylcoba ...
Caenorhabditis elegans
Mol. Genet. Metab.
89
64-73
2006
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1
1
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1
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661687
Kühnl
Functional analysis of the met ...
Caenorhabditis elegans
FEBS J.
272
1465-1477
2005
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649408
Bobik
Purification and partial chara ...
Pyrococcus horikoshii
Appl. Microbiol. Biotechnol.
63
682-685
2004
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1
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1
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1
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4
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1
1
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1
1
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649165
McCarthy
Expression, crystallization an ...
Propionibacterium freudenreichii subsp. shermanii
Acta Crystallogr. Sect. D
57
706-708
2001
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1
1
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653930
McCarthy
Crystal structure of methylmal ...
Propionibacterium freudenreichii subsp. shermanii
Structure
9
637-646
2001
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1
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5
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2442
Stabler
DL-Methylmalonyl-CoA racemase ...
Rattus norvegicus
Methods Enzymol.
166
400-406
1988
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4
1
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4
2
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2
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1
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1
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2434
Stabler
Isolation and characterization ...
Rattus norvegicus
Arch. Biochem. Biophys.
241
252-264
1985
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1
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13
1
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1
1
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13
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2
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1
1
2
1
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1
1
1
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2435
Fuller
Proton transfer in methylmalon ...
Homo sapiens, Propionibacterium freudenreichii subsp. shermanii
Biochem. J.
213
643-650
1983
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2436
Leadlay
Proton transfer in methylmalon ...
Propionibacterium freudenreichii subsp. shermanii
Biochem. J.
213
635-642
1983
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2437
Leadlay
Purification and characterizat ...
Propionibacterium freudenreichii subsp. shermanii
Biochem. J.
197
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1981
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5
2
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2
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2438
Allen
-
Methylmalonyl-CoA racemase fro ...
Propionibacterium freudenreichii subsp. shermanii
Methods Enzymol.
13
194-198
1969
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2439
Mazumder
-
Methylmalonyl-CoA racemase fro ...
Ovis aries
Methods Enzymol.
13
190-194
1969
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1
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2440
Overath
-
Zum Mechanismus der Umlagerung ...
Propionibacterium freudenreichii subsp. shermanii
Biochem. Z.
335
500-518
1962
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2441
Mazumder
Metabolism of propionic acid i ...
Ovis aries
J. Biol. Chem.
237
3065-3068
1962
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2
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