Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Metallosphaera sedula |
gene Msed_0639, DNA and amino acid sequence determination, phylogenetic tree, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain Rosetta 2 (DE3) | Metallosphaera sedula |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates | Metallosphaera sedula | |
Co2+ | the enzyme is activated by divalent cations (Ni2+, Co2+, and Mg2+) | Metallosphaera sedula | |
Mg2+ | activates | Metallosphaera sedula | |
Mg2+ | the enzyme is activated by divalent cations (Ni2+, Co2+, and Mg2+) | Metallosphaera sedula | |
Ni2+ | activates | Metallosphaera sedula | |
Ni2+ | the enzyme is activated by divalent cations (Ni2+, Co2+, and Mg2+) | Metallosphaera sedula |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
2 * 20000, SDS-PAGE | Metallosphaera sedula |
20000 | - |
2 * 20000, recombinant His-tagged enzyme, SDS-PAGE | Metallosphaera sedula |
32100 | - |
gel filtration | Metallosphaera sedula |
32100 | - |
recombinant His-tagged enzyme, gel filtration | Metallosphaera sedula |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-methylmalonyl-CoA | Metallosphaera sedula | - |
(R)-methylmalonyl-CoA | - |
? | |
(S)-methylmalonyl-CoA | Metallosphaera sedula | the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway | (R)-methylmalonyl-CoA | - |
? | |
(S)-methylmalonyl-CoA | Metallosphaera sedula DSM 5348 | - |
(R)-methylmalonyl-CoA | - |
? | |
(S)-methylmalonyl-CoA | Metallosphaera sedula DSM 5348 | the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway | (R)-methylmalonyl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Metallosphaera sedula | A4YEG2 | - |
- |
Metallosphaera sedula DSM 5348 | A4YEG2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Metallosphaera sedula |
recombinant N-terminally His-tagged enzyme from Escherichia coli strain Rosetta 2 (DE3) by nickel affinity chromatography | Metallosphaera sedula |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | transcriptomic analyses of Metallosphaera sedula grown under autotrophic versus heterotrophic conditions, overview. MCE expression is significantly upregulated under autotrophic compared to heterotrophic growth conditions | Metallosphaera sedula | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
218 | - |
75°C, pH not specified in the publication | Metallosphaera sedula |
218 | - |
purified recombinant enzyme, pH 7.0, 75°C | Metallosphaera sedula |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-methylmalonyl-CoA | - |
Metallosphaera sedula | (R)-methylmalonyl-CoA | - |
? | |
(S)-methylmalonyl-CoA | the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway | Metallosphaera sedula | (R)-methylmalonyl-CoA | - |
? | |
(S)-methylmalonyl-CoA | - |
Metallosphaera sedula DSM 5348 | (R)-methylmalonyl-CoA | - |
? | |
(S)-methylmalonyl-CoA | the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway | Metallosphaera sedula DSM 5348 | (R)-methylmalonyl-CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 20000, SDS-PAGE | Metallosphaera sedula |
homodimer | 2 * 20000, recombinant His-tagged enzyme, SDS-PAGE | Metallosphaera sedula |
More | the putative three-dimensional structure of the enzyme is modeled using hypothetical protein BH1468, PDB ID 3OA4 | Metallosphaera sedula |
Synonyms | Comment | Organism |
---|---|---|
MCE | - |
Metallosphaera sedula |
methylmalonyl-CoA epimerase | - |
Metallosphaera sedula |
methylmalonyl-coenzyme A epimerase | - |
Metallosphaera sedula |
Msed_0639 | locus name | Metallosphaera sedula |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
73 | - |
- |
Metallosphaera sedula |
75 | - |
assay at | Metallosphaera sedula |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Metallosphaera sedula |
Organism | Comment | Expression |
---|---|---|
Metallosphaera sedula | expression of the gene is significantly upregulated under autotrophic compared to heterotrophic growth conditions, implying a role in CO2 fixation | up |
Metallosphaera sedula | the enzyme expression is significantly upregulated under autotrophic compared to heterotrophic growth conditions | up |
General Information | Comment | Organism |
---|---|---|
physiological function | methylmalonyl-CoA epimerase and methylmalonyl-CoA mutase are responsible for producing succinyl-CoA from (S)-methylmalonyl-CoA. Succinyl-CoA is an intermediate connecting the 3-hydroxypropionate/4-hydroxybutyrate cycle and general carbon metabolism. Methylmalonyl-CoA epimerase and methylmalonyl-CoA mutase may also be involved in degradation of propionate and branched-chain amino acids (valine, isoleucine, methionine). The enzyme has a role in CO2 fixation | Metallosphaera sedula |
physiological function | the enzyme is involved in the 3-hydroxypropionate/4-hydroxybutyrate carbon fixation pathway | Metallosphaera sedula |