Cloned (Comment) | Organism |
---|---|
recombinant protein is expressed in BL21(DE3) Escherichia coli cells | Caldanaerobacter subterraneus subsp. tengcongensis |
Crystallization (Comment) | Organism |
---|---|
at 2.0 A resolutionm, crystals of recombinant enzyme are grown at 20°C using the hanging-drop, vapor-diffusion method, drops consisted of 2 microl of protein solution and 2 microl of mother liquor (0.1 M citric acid, pH 2.6, 1.6 M (NH4)2SO4), crystals are obtained after 7-8 days growth, native and derivative crystals are soaked in 2 M Li2SO4 | Caldanaerobacter subterraneus subsp. tengcongensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | enzyme is metal-dependent enzyme, in the active site, metal binding site binds the coenzyme Co2+ | Caldanaerobacter subterraneus subsp. tengcongensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-methylmalonyl-CoA | Caldanaerobacter subterraneus subsp. tengcongensis | - |
(S)-methylmalonyl-CoA | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caldanaerobacter subterraneus subsp. tengcongensis | Q8RCQ6 | - |
- |
Purification (Comment) | Organism |
---|---|
lysate is clarified by centrifugation and purified by passage through a nickel-affinity column, and a further purification step is performed by gel filtration | Caldanaerobacter subterraneus subsp. tengcongensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-methylmalonyl-CoA | - |
Caldanaerobacter subterraneus subsp. tengcongensis | (S)-methylmalonyl-CoA | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | in solution | Caldanaerobacter subterraneus subsp. tengcongensis |
Synonyms | Comment | Organism |
---|---|---|
methylmalonyl-CoA epimerase | - |
Caldanaerobacter subterraneus subsp. tengcongensis |
MMCE | - |
Caldanaerobacter subterraneus subsp. tengcongensis |
TTE0360 | - |
Caldanaerobacter subterraneus subsp. tengcongensis |
General Information | Comment | Organism |
---|---|---|
physiological function | second step in the pathway responsible for the degradation of branched amino acids and odd chain fatty acids, MMCE participated in autotrophic CO2 fixation via the 3-hydroxypropionate pathway, in propionate fermentation, the regeneration of glyoxylate and in the biosynthesis of polyketide antibiotics | Caldanaerobacter subterraneus subsp. tengcongensis |