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Literature summary for 5.1.99.1 extracted from

  • Bobik, T.A.; Rasche, M.E.
    Purification and partial characterization of the Pyrococcus horikoshii methylmalonyl-CoA epimerase (2004), Appl. Microbiol. Biotechnol., 63, 682-685.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
high level expression in Escherichia coli Pyrococcus horikoshii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.079
-
methylmalonyl-CoA
-
Pyrococcus horikoshii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31700
-
2 * 31700 Pyrococcus horikoshii

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-methylmalonyl-CoA
-
Pyrococcus horikoshii (S)-methylmalonyl-CoA
-
?
methylmalonyl-CoA
-
Pyrococcus horikoshii ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 31700 Pyrococcus horikoshii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
60 min, stable Pyrococcus horikoshii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
240
-
methylmalonyl-CoA
-
Pyrococcus horikoshii