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Literature summary for 5.1.99.1 extracted from
- Purification and characterization of methylmalonyl-CoA epimerase from Propionibacterium shermanii (1981), Biochem. J., 197, 413-419.
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | increases activity | Propionibacterium freudenreichii subsp. shermanii |  |
| Mn2+ | increases activity to a smaller extent than Co2+ | Propionibacterium freudenreichii subsp. shermanii | |
| additional information | enzyme does not require tightly bound metal ions for activity, they are not released even after extensive dialysis at pH 2.4 | Propionibacterium freudenreichii subsp. shermanii | |
| Ni2+ | increases activity to a smaller extent than Co2+ | Propionibacterium freudenreichii subsp. shermanii | |
| Zn2+ | increases activity to a smaller extent than Co2+ | Propionibacterium freudenreichii subsp. shermanii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17000 | - |
2 * 17000, SDS-PAGE in presence of 2-mercaptoethanol | Propionibacterium freudenreichii subsp. shermanii |
| 33000 | 35000 | gel filtration, sedimentation equilibrium studies | Propionibacterium freudenreichii subsp. shermanii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Propionibacterium freudenreichii subsp. shermanii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Propionibacterium freudenreichii subsp. shermanii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Propionibacterium freudenreichii subsp. shermanii |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, pH 8.5, indefinitely stable | Propionibacterium freudenreichii subsp. shermanii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-2-Methyl-3-oxopropanoyl-CoA | - |
Propionibacterium freudenreichii subsp. shermanii | (S)-2-Methyl-3-oxopropanoyl-CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 17000, SDS-PAGE in presence of 2-mercaptoethanol | Propionibacterium freudenreichii subsp. shermanii |
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