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Literature summary for 5.1.99.1 extracted from
- Isolation and characterization of DL-methylmalonyl-coenzyme A racemase from rat liver (1985), Arch. Biochem. Biophys., 241, 252-264.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | a simple direct assay for DL-methylmalonyl-coenzyme A racemase which is based on the fact that the proton on C-2 of methylmalonyl-CoA is replaced by a proton in the medium during racemization | Rattus norvegicus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetyl-CoA | - |
Rattus norvegicus |  |
| butyryl-CoA | - |
Rattus norvegicus | |
| Cd2+ | - |
Rattus norvegicus | |
| CoA | - |
Rattus norvegicus | |
| Cu2+ | - |
Rattus norvegicus | |
| EDTA | reactivation by Co2+ | Rattus norvegicus | |
| glutaryl-CoA | - |
Rattus norvegicus | |
| heptanoyl-CoA | - |
Rattus norvegicus | |
| malonyl-CoA | - |
Rattus norvegicus | |
| octanoyl-CoA | - |
Rattus norvegicus | |
| propionyl-CoA | - |
Rattus norvegicus | |
| succinyl-CoA | - |
Rattus norvegicus | |
| Zn2+ | - |
Rattus norvegicus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
DL-methylmalonyl-CoA | - |
Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | most potent activator, Km: 0.0002 mM, binds 2 mol Co2+ per mol of racemase | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 16000 | - |
2 * 16000, subunits not connected by disulfide bonds, SDS-PAGE in presence and absence of 2-mercaptoethanol | Rattus norvegicus |
| 32000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Rattus norvegicus | enzyme is involved in the flow of DL-methylmalonyl-CoA to L-methylmalonyl-CoA | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8400 | - |
- |
Rattus norvegicus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for at least 3 months | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-2-Methyl-3-oxopropanoyl-CoA | - |
Rattus norvegicus | (S)-2-Methyl-3-oxopropanoyl-CoA | - |
? | |
| DL-methylmalonyl-CoA | - |
Rattus norvegicus | ? | - |
? | |
| additional information | enzyme is involved in the flow of DL-methylmalonyl-CoA to L-methylmalonyl-CoA | Rattus norvegicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 16000, subunits not connected by disulfide bonds, SDS-PAGE in presence and absence of 2-mercaptoethanol | Rattus norvegicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4170 | - |
DL-methylmalonyl-CoA | - |
Rattus norvegicus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Rattus norvegicus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8.5 | 50% of maximal activity at pH 5.5 and 8.5 | Rattus norvegicus |
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Release 2023.1 (January 2023)
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