Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | allosteric activation | Acaryochloris marina | |
ATP | allosteric activation, ATP has a more pronounced effect on the Km for the substrate than on the enzyme activity. The enzyme shows 20% of maximal activity without ATP. At high substrate concentrations of 200 mM and without ATP, the enzyme reaches up to 32% of the activity measured with ATP in excess | Trichormus variabilis | |
ATP | allosteric activation, the enzyme shows 13% of maximal activity without ATP | Nostoc sp. | |
ATP | allosteric activation, the enzyme shows 18% of maximal activity without ATP | Nostoc punctiforme |
Cloned (Comment) | Organism |
---|---|
expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) in inclusion bodies, subcloning in Escherichia coli strain DH5alpha | Acaryochloris marina |
expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | Trichormus variabilis |
expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | Nostoc sp. |
expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | Nostoc punctiforme |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-acetylneuraminic acid | linear competitive with respect to GlcNAc, kinetics of the reversible uni-uni reaction, overview | Nostoc punctiforme | |
N-acetylneuraminic acid | linear competitive with respect to GlcNAc, kinetics of the reversible uni-uni reaction, overview | Nostoc sp. | |
N-acetylneuraminic acid | linear competitive with respect to GlcNAc, kinetics of the reversible uni-uni reaction, overview | Trichormus variabilis | |
pyruvate | - |
Acaryochloris marina | |
pyruvate | linear competitive with respect to GlcNAc, kinetics of the reversible uni-uni reaction, overview | Nostoc punctiforme | |
pyruvate | linear competitive with respect to GlcNAc, kinetics of the reversible uni-uni reaction, overview | Nostoc sp. | |
pyruvate | low inhibition, linear competitive with respect to GlcNAc, kinetics of the reversible uni-uni reaction, overview | Trichormus variabilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | 2.2 | N-acetyl-D-glucosamine | pH 9.0, 30°C | Trichormus variabilis | |
16.3 | - |
N-acetyl-D-glucosamine | pH 7.5, 30°C | Trichormus variabilis | |
16.6 | - |
N-acetyl-D-glucosamine | pH 7.5, 30°C | Nostoc punctiforme | |
16.6 | - |
N-acetyl-D-mannosamine | pH 7.5, 30°C | Nostoc punctiforme | |
16.7 | - |
N-acetyl-D-mannosamine | pH 7.5, 30°C | Trichormus variabilis | |
18.8 | - |
N-acetyl-D-glucosamine | pH 7.5, 37°C | Trichormus variabilis | |
18.8 | - |
N-acetyl-D-glucosamine | pH 8.0, 30°C | Trichormus variabilis | |
19.7 | - |
N-acetyl-D-mannosamine | pH 7.5, 30°C | Nostoc sp. | |
24.6 | - |
N-acetyl-D-glucosamine | pH 7.5, 30°C | Nostoc sp. | |
26.1 | - |
N-acetyl-D-glucosamine | pH 7.0, 30°C | Trichormus variabilis | |
62.5 | - |
N-acetyl-D-glucosamine | pH 6.0, 30°C | Trichormus variabilis | |
113.9 | - |
N-acetyl-D-glucosamine | pH 7.5, 30°C, without ATP | Trichormus variabilis | |
231.9 | - |
N-acetyl-D-glucosamine | pH 7.5, 37°C, without ATP | Trichormus variabilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-D-glucosamine | Trichormus variabilis | - |
N-acetyl-D-mannosamine | - |
r | |
N-acetyl-D-glucosamine | Nostoc sp. | - |
N-acetyl-D-mannosamine | - |
r | |
N-acetyl-D-glucosamine | Nostoc punctiforme | - |
N-acetyl-D-mannosamine | - |
r | |
N-acetyl-D-glucosamine | Acaryochloris marina | - |
N-acetyl-D-mannosamine | - |
r | |
N-acetyl-D-glucosamine | Nostoc sp. PCC 7120 | - |
N-acetyl-D-mannosamine | - |
r | |
N-acetyl-D-glucosamine | Acaryochloris marina MBIC 11017 | - |
N-acetyl-D-mannosamine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acaryochloris marina | - |
- |
- |
Acaryochloris marina MBIC 11017 | - |
- |
- |
Nostoc punctiforme | - |
- |
- |
Nostoc sp. | - |
- |
- |
Nostoc sp. PCC 7120 | - |
- |
- |
Trichormus variabilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis | Trichormus variabilis |
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis | Nostoc sp. |
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis | Nostoc punctiforme |
Renatured (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme expressed in Escherichia coli strain BL21(DE3) cannot be solubilized/purified from inclusion bodies | Acaryochloris marina |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
79.2 | - |
with N-acetyl-D-glucosamine, pH 6.0, 30°C | Trichormus variabilis |
82.1 | - |
with N-acetyl-D-glucosamine, pH 7.0, 30°C | Trichormus variabilis |
82.4 | - |
with 250 mM N-acetyl-D-glucosamine, pH 9.0, 30°C | Trichormus variabilis |
90.8 | - |
with N-acetyl-D-glucosamine, pH 8.0, 30°C | Trichormus variabilis |
117 | - |
with N-acetyl-D-glucosamine, pH 7.5, 30°C | Trichormus variabilis |
131 | - |
with 430 mM N-acetyl-D-glucosamine, pH 9.0, 30°C | Trichormus variabilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-D-glucosamine | - |
Trichormus variabilis | N-acetyl-D-mannosamine | - |
r | |
N-acetyl-D-glucosamine | - |
Nostoc sp. | N-acetyl-D-mannosamine | - |
r | |
N-acetyl-D-glucosamine | - |
Nostoc punctiforme | N-acetyl-D-mannosamine | - |
r | |
N-acetyl-D-glucosamine | - |
Acaryochloris marina | N-acetyl-D-mannosamine | - |
r | |
N-acetyl-D-glucosamine | - |
Nostoc sp. PCC 7120 | N-acetyl-D-mannosamine | - |
r | |
N-acetyl-D-glucosamine | - |
Acaryochloris marina MBIC 11017 | N-acetyl-D-mannosamine | - |
r | |
N-acetyl-D-mannosamine | - |
Trichormus variabilis | N-acetyl-D-glucosamine | - |
r | |
N-acetyl-D-mannosamine | - |
Nostoc sp. | N-acetyl-D-glucosamine | - |
r | |
N-acetyl-D-mannosamine | - |
Nostoc punctiforme | N-acetyl-D-glucosamine | - |
r | |
N-acetyl-D-mannosamine | - |
Nostoc sp. PCC 7120 | N-acetyl-D-glucosamine | - |
r |
Synonyms | Comment | Organism |
---|---|---|
AGE | - |
Trichormus variabilis |
AGE | - |
Nostoc sp. |
AGE | - |
Nostoc punctiforme |
AGE | - |
Acaryochloris marina |
N-acyl-D-glucosamine 2-epimerase | - |
Trichormus variabilis |
N-acyl-D-glucosamine 2-epimerase | - |
Nostoc sp. |
N-acyl-D-glucosamine 2-epimerase | - |
Nostoc punctiforme |
N-acyl-D-glucosamine 2-epimerase | - |
Acaryochloris marina |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Trichormus variabilis |
30 | - |
assay at | Nostoc sp. |
30 | - |
assay at | Nostoc punctiforme |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Trichormus variabilis |
7.5 | - |
assay at | Nostoc sp. |
7.5 | - |
assay at | Nostoc punctiforme |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
14.7 | - |
N-acetylneuraminic acid | pH 7.5, 30°C | Nostoc sp. | |
15.1 | - |
N-acetylneuraminic acid | pH 7.5, 30°C | Nostoc punctiforme | |
38.7 | - |
N-acetylneuraminic acid | pH 7.5, 30°C | Trichormus variabilis | |
412 | - |
pyruvate | pH 7.5, 30°C | Trichormus variabilis | |
454 | - |
pyruvate | pH 7.5, 30°C | Nostoc sp. | |
463 | - |
pyruvate | pH 7.5, 30°C | Nostoc punctiforme |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.17 | - |
N-acetyl-D-glucosamine | pH 7.5, 30°C | Trichormus variabilis | |
3.15 | - |
N-acetyl-D-glucosamine | pH 6.0, 30°C | Trichormus variabilis | |
3.26 | - |
N-acetyl-D-glucosamine | pH 8.0, 30°C | Trichormus variabilis | |
4.96 | - |
N-acetyl-D-glucosamine | pH 7.0, 30°C | Trichormus variabilis |