Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ADP | lower activating effect compared to ATP | Bacteroides ovatus | |
ATP | activates isozyme AGE2, incubation with ATP stabilized its structure, raising its melting temperature by about 8 degrees | Bacteroides ovatus | |
dATP | lower activating effect compared to ATP | Bacteroides ovatus | |
dCTP | low activating effect | Bacteroides ovatus | |
dTTP | low activating effect | Bacteroides ovatus | |
renin | activates isozyme BoAGE2, unrelatedly with the unique native N-terminal tag, an addiional 27-amino acid tag sequence, detagged enzyme is also activated by renin. BoAGE2 can be considered a renin-binding protein | Bacteroides ovatus |
Application | Comment | Organism |
---|---|---|
synthesis | BoAGE2 a promising biocatalyst for sialic acid production using cheap GlcNAc as starting material | Bacteroides ovatus |
Cloned (Comment) | Organism |
---|---|
gene BACOVA_01816, expression of His-tagged enzyme in Escherichia coli strain Rosetta (DE3)pLys | Bacteroides ovatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | slight inhibition | Bacteroides ovatus | |
dGTP | - |
Bacteroides ovatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-Acyl-D-glucosamine | Bacteroides ovatus | - |
N-Acyl-D-mannosamine | - |
r | |
N-Acyl-D-glucosamine | Bacteroides ovatus ATCC 8483 | - |
N-Acyl-D-mannosamine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacteroides ovatus | A7LVG6 | gene BACOVA_01816 | - |
Bacteroides ovatus ATCC 8483 | A7LVG6 | gene BACOVA_01816 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain Rosetta (DE3)pLys by ultrafiltration and nickel affinityy chromatography | Bacteroides ovatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-D-mannosamine | activity of isozyme AGE2, catalytic efficiency for ManNAc synthesis of the isozyme is higher than that for GlcNAc synthesis, coupled assay with N-acyl-D-mannosamine dehydrogenase, EC 1.1.1.233 | Bacteroides ovatus | N-acetyl-D-glucosamine | - |
r | |
N-acetyl-D-mannosamine | activity of isozyme AGE2, catalytic efficiency for ManNAc synthesis of the isozyme is higher than that for GlcNAc synthesis, coupled assay with N-acyl-D-mannosamine dehydrogenase, EC 1.1.1.233 | Bacteroides ovatus ATCC 8483 | N-acetyl-D-glucosamine | - |
r | |
N-Acyl-D-glucosamine | - |
Bacteroides ovatus | N-Acyl-D-mannosamine | - |
r | |
N-Acyl-D-glucosamine | - |
Bacteroides ovatus ATCC 8483 | N-Acyl-D-mannosamine | - |
r |
Subunits | Comment | Organism |
---|---|---|
More | three-dimensional structure modeling, overview | Bacteroides ovatus |
Synonyms | Comment | Organism |
---|---|---|
AGE | - |
Bacteroides ovatus |
AGE2 | - |
Bacteroides ovatus |
N-acyl-D-glucosamine 2-epimerase | - |
Bacteroides ovatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
both reaction directions | Bacteroides ovatus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 60 | activity range, activity increases up to 60°C | Bacteroides ovatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
both reaction directions | Bacteroides ovatus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9.5 | activity range in both reaction directions | Bacteroides ovatus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.5 | 50% activity remaining within this range after 24h | Bacteroides ovatus |
General Information | Comment | Organism |
---|---|---|
evolution | AGE enzymes can be divided in 3 different groups,overview | Bacteroides ovatus |
additional information | three-dimensional structure modeling, overview | Bacteroides ovatus |