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Literature summary for 5.1.3.8 extracted from

  • Sola-Carvajal, A.; Sanchez-Carron, G.; Garcia-Garcia, M.I.; Garcia-Carmona, F.; Sanchez-Ferrer, A.
    Properties of BoAGE2, a second N-acetyl-D-glucosamine 2-epimerase from Bacteroides ovatus ATCC 8483 (2012), Biochimie, 94, 222-230.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ADP lower activating effect compared to ATP Bacteroides ovatus
ATP activates isozyme AGE2, incubation with ATP stabilized its structure, raising its melting temperature by about 8 degrees Bacteroides ovatus
dATP lower activating effect compared to ATP Bacteroides ovatus
dCTP low activating effect Bacteroides ovatus
dTTP low activating effect Bacteroides ovatus
renin activates isozyme BoAGE2, unrelatedly with the unique native N-terminal tag, an addiional 27-amino acid tag sequence, detagged enzyme is also activated by renin. BoAGE2 can be considered a renin-binding protein Bacteroides ovatus

Application

Application Comment Organism
synthesis BoAGE2 a promising biocatalyst for sialic acid production using cheap GlcNAc as starting material Bacteroides ovatus

Cloned(Commentary)

Cloned (Comment) Organism
gene BACOVA_01816, expression of His-tagged enzyme in Escherichia coli strain Rosetta (DE3)pLys Bacteroides ovatus

Inhibitors

Inhibitors Comment Organism Structure
AMP slight inhibition Bacteroides ovatus
dGTP
-
Bacteroides ovatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-Acyl-D-glucosamine Bacteroides ovatus
-
N-Acyl-D-mannosamine
-
r
N-Acyl-D-glucosamine Bacteroides ovatus ATCC 8483
-
N-Acyl-D-mannosamine
-
r

Organism

Organism UniProt Comment Textmining
Bacteroides ovatus A7LVG6 gene BACOVA_01816
-
Bacteroides ovatus ATCC 8483 A7LVG6 gene BACOVA_01816
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain Rosetta (DE3)pLys by ultrafiltration and nickel affinityy chromatography Bacteroides ovatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-D-mannosamine activity of isozyme AGE2, catalytic efficiency for ManNAc synthesis of the isozyme is higher than that for GlcNAc synthesis, coupled assay with N-acyl-D-mannosamine dehydrogenase, EC 1.1.1.233 Bacteroides ovatus N-acetyl-D-glucosamine
-
r
N-acetyl-D-mannosamine activity of isozyme AGE2, catalytic efficiency for ManNAc synthesis of the isozyme is higher than that for GlcNAc synthesis, coupled assay with N-acyl-D-mannosamine dehydrogenase, EC 1.1.1.233 Bacteroides ovatus ATCC 8483 N-acetyl-D-glucosamine
-
r
N-Acyl-D-glucosamine
-
Bacteroides ovatus N-Acyl-D-mannosamine
-
r
N-Acyl-D-glucosamine
-
Bacteroides ovatus ATCC 8483 N-Acyl-D-mannosamine
-
r

Subunits

Subunits Comment Organism
More three-dimensional structure modeling, overview Bacteroides ovatus

Synonyms

Synonyms Comment Organism
AGE
-
Bacteroides ovatus
AGE2
-
Bacteroides ovatus
N-acyl-D-glucosamine 2-epimerase
-
Bacteroides ovatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
both reaction directions Bacteroides ovatus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 60 activity range, activity increases up to 60°C Bacteroides ovatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
both reaction directions Bacteroides ovatus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.5 activity range in both reaction directions Bacteroides ovatus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 8.5 50% activity remaining within this range after 24h Bacteroides ovatus

General Information

General Information Comment Organism
evolution AGE enzymes can be divided in 3 different groups,overview Bacteroides ovatus
additional information three-dimensional structure modeling, overview Bacteroides ovatus