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Literature summary for 5.1.3.8 extracted from

  • Zhang, Y.; Tao, F.; Du, M.; Ma, C.; Qiu, J.; Gu, L.; He, X.; Xu, P.
    An efficient method for N-acetyl-D-neuraminic acid production using coupled bacterial cells with a safe temperature-induced system (2010), Appl. Microbiol. Biotechnol., 86, 481-489.
    View publication on PubMed

Application

Application Comment Organism
biotechnology efficient method for N-acetyl-D-neuraminic acid production using coupled bacterial cells with a safe temperature-induced system, a precursor for producing many pharmaceutical drugs such as zanamivir which have been used in clinical trials to treat and prevent the infection with influenza virus, such as the avian influenza virus H5N1 and the current 2009 H1N1 Synechocystis sp. PCC 6803

Cloned(Commentary)

Cloned (Comment) Organism
2 recombinant Escherichia coli strains capable of expressing N-acetyl-D-glucosamine 2-epimerase and N-acetyl-D-neuraminic acid aldolase are constructed based on a highly efficient temperature-responsive expression system which is safe compared to chemical-induced systems and coupled in N-acetyl-D-neuraminic acid production Synechocystis sp. PCC 6803

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
determined by SDS-PAGE Synechocystis sp. PCC 6803

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-D-mannosamine Synechocystis sp. PCC 6803
-
N-acetyl-D-glucosamine
-
r

Organism

Organism UniProt Comment Textmining
Synechocystis sp. PCC 6803
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-D-mannosamine
-
Synechocystis sp. PCC 6803 N-acetyl-D-glucosamine
-
r

Synonyms

Synonyms Comment Organism
AGE
-
Synechocystis sp. PCC 6803
GlcNAc 2-epimerase
-
Synechocystis sp. PCC 6803
N-acetyl-D-glucosamine 2-epimerase
-
Synechocystis sp. PCC 6803

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Synechocystis sp. PCC 6803

Cofactor

Cofactor Comment Organism Structure
ATP necessary activator of the GlcNAc 2-epimerase Synechocystis sp. PCC 6803