Application | Comment | Organism |
---|---|---|
biotechnology | efficient method for N-acetyl-D-neuraminic acid production using coupled bacterial cells with a safe temperature-induced system, a precursor for producing many pharmaceutical drugs such as zanamivir which have been used in clinical trials to treat and prevent the infection with influenza virus, such as the avian influenza virus H5N1 and the current 2009 H1N1 | Synechocystis sp. PCC 6803 |
Cloned (Comment) | Organism |
---|---|
2 recombinant Escherichia coli strains capable of expressing N-acetyl-D-glucosamine 2-epimerase and N-acetyl-D-neuraminic acid aldolase are constructed based on a highly efficient temperature-responsive expression system which is safe compared to chemical-induced systems and coupled in N-acetyl-D-neuraminic acid production | Synechocystis sp. PCC 6803 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
determined by SDS-PAGE | Synechocystis sp. PCC 6803 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-D-mannosamine | Synechocystis sp. PCC 6803 | - |
N-acetyl-D-glucosamine | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. PCC 6803 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-D-mannosamine | - |
Synechocystis sp. PCC 6803 | N-acetyl-D-glucosamine | - |
r |
Synonyms | Comment | Organism |
---|---|---|
AGE | - |
Synechocystis sp. PCC 6803 |
GlcNAc 2-epimerase | - |
Synechocystis sp. PCC 6803 |
N-acetyl-D-glucosamine 2-epimerase | - |
Synechocystis sp. PCC 6803 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Synechocystis sp. PCC 6803 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | necessary activator of the GlcNAc 2-epimerase | Synechocystis sp. PCC 6803 |