Cloned (Comment) | Organism |
---|---|
construction of several C-terminal deletion and multi-cysteine/serine mutants and expression in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C125S | no loss of activity compared to wild-type enzyme | Homo sapiens |
C125S/C210S | mutant enzyme shows 54.8% of the activity relative to the wild-type enzyme | Homo sapiens |
C125S/C210S/C239S | mutant enzyme shows 49.3% of the activity relative to the wild-type enzyme | Homo sapiens |
C125S/C210S/C239S/C203S | mutant enzyme shows 28.7% of the activity relative to the wild-type enzyme | Homo sapiens |
C125S/C210S/C239S/C203S/C386S | mutant enzyme shows 23.8% of the activity relative to the wild-type enzyme | Homo sapiens |
C125S/C210S/C302S/C390S | no activity detected | Homo sapiens |
C125S/C386S | mutant enzyme shows 68.7% of the activity relative to the wild-type enzyme | Homo sapiens |
C125S/C390S | mutant enzyme shows 5.1% of the activity relative to the wild-type enzyme | Homo sapiens |
C210S/C386S | mutant enzyme shows 88.7% of the activity relative to the wild-type enzyme | Homo sapiens |
C210S/C390S | mutant enzyme shows 30.9% of the activity relative to the wild-type enzyme | Homo sapiens |
C239S/C386S | mutant enzyme shows 116% of the activity relative to the wild-type enzyme | Homo sapiens |
C239S/C390S | mutant enzyme shows 27.8% of the activity relative to the wild-type enzyme | Homo sapiens |
C302S/C386S | mutant enzyme shows 65.8% of the activity relative to the wild-type enzyme | Homo sapiens |
C302S/C390S | mutant enzyme shows 7.4% of the activity relative to the wild-type enzyme | Homo sapiens |
C386S | no loss of activity compared to wild-type enzyme | Homo sapiens |
C390S | no loss of activity compared to wild-type enzyme | Homo sapiens |
C41S | no loss of activity compared to wild-type enzyme | Homo sapiens |
C41S/C125S | mutant enzyme shows 17.7% of the activity relative to the wild-type enzyme | Homo sapiens |
C41S/C125S/C210S | mutant enzyme shows 28.1% of the activity relative to the wild-type enzyme | Homo sapiens |
C41S/C125S/C210S/C239S | mutant enzyme shows 9.7% of the activity relative to the wild-type enzyme | Homo sapiens |
C41S/C125S/C210S/C239S/C302S | no activity detected | Homo sapiens |
C41S/C125S/C210S/C239S/C302S/C386S | no activity detected | Homo sapiens |
C41S/C125S/C210S/C239S/C302S/C390S | no activity detected | Homo sapiens |
C41S/C386S | mutant enzyme shows 0.7% of the activity relative to the wild-type enzyme | Homo sapiens |
C41S/C390S | no activity detected | Homo sapiens |
C66S | no loss of activity compared to wild-type enzyme | Homo sapiens |
C66S/C125S | mutant enzyme shows 39.4% of the activity relative to the wild-type enzyme | Homo sapiens |
C66S/C125S/C210S | mutant enzyme shows 23.9% of the activity relative to the wild-type enzyme | Homo sapiens |
C66S/C125S/C210S/C239S | mutant enzyme shows 58.4% of the activity relative to the wild-type enzyme | Homo sapiens |
C66S/C125S/C210S/C239S/C302S | mutant enzyme shows 15.5% of the activity relative to the wild-type enzyme | Homo sapiens |
C66S/C125S/C210S/C302S/C386S | no activity detected | Homo sapiens |
C66S/C125S/C210S/C302S/C390S | no activity detected | Homo sapiens |
C66S/C386S | mutant enzyme shows 113% of the activity relative to the wild-type enzyme | Homo sapiens |
C66S/C390S | mutant enzyme shows 14.4% of the activity relative to the wild-type enzyme | Homo sapiens |
DELTA380-417 | mutant enzyme has no activity | Homo sapiens |
DELTA386-417 | mutant enzyme has no activity | Homo sapiens |
DELTA390-417 | mutant enzyme has no activity | Homo sapiens |
DELTA400-417 | C-terminal deletion mutant has approximately 50% activity relative to the wild-type enzyme | Homo sapiens |
additional information | mutational analysis of multi-cysteine/serine mutants reveals that Cys41 and Cys390 are critical for the activity or stabilization of the enzyme, while cysteine residues in the middle of the enzyme, Cys125, Cys210, Cys239, and Cys302 have no essential function in relation to the activity | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-D-mannosamine | - |
Homo sapiens | N-acetyl-D-glucosamine | - |
? |