Cloned (Comment) | Organism |
---|---|
expression of wild-type enzyme and mutant enzymes C41S, C66S, C104S, C125S, C210S, C239S, C302S, C380S, C386S and C390S in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C104S | specific activity in the extract is 25% of that of the wild-type enzyme | Homo sapiens |
C125S | relative specific activity in the extract is nearly the same to that of the wild-type enzyme | Homo sapiens |
C210S | relative specific activity in the extract is nearly the same to that of the wild-type enzyme | Homo sapiens |
C239S | relative specific activity in the extract is nearly the same to that of the wild-type enzyme | Homo sapiens |
C302S | relative specific activity in the extract is nearly the same to that of the wild-type enzyme | Homo sapiens |
C380S | no enzyme activity detected in the extract | Homo sapiens |
C386S | relative specific activity in the extract is nearly the same to that of the wild-type enzyme | Homo sapiens |
C390S | relative specific activity in the extract is nearly the same to that of the wild-type enzyme | Homo sapiens |
C41S | relative specific activity in the extract is nearly the same to that of the wild-type enzyme | Homo sapiens |
C66S | relative specific activity in the extract is nearly the same to that of the wild-type enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis-2-nitrobenzoate | 0.1 mM, more than 95% inhibition | Homo sapiens | |
Monoiodoacetic acid | 10 mM, more than 95% inhibition | Homo sapiens | |
NaN3 | 10 mM, 18% inhibition | Homo sapiens | |
NEM | 1 mM, more than 95% inhibition | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
82000 | - |
gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-D-mannosamine | - |
Homo sapiens | N-acetyl-D-glucosamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
renin binding protein | - |
Homo sapiens |
RNBP | - |
Homo sapiens |