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Literature summary for 5.1.3.8 extracted from

  • Takahashi, S.; Takahashi, K.; Kaneko, T.; Ogasawara, H.; Shindo, S.; Saito, K.; Kobayashi, M.
    Identification of cysteine-380 as the essential residue for the human N-acetyl-D-glucosamine 2-epimerase (renin binding protein) (1999), J. Biochem., 126, 639-642.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type enzyme and mutant enzymes C41S, C66S, C104S, C125S, C210S, C239S, C302S, C380S, C386S and C390S in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
C104S specific activity in the extract is 25% of that of the wild-type enzyme Homo sapiens
C125S relative specific activity in the extract is nearly the same to that of the wild-type enzyme Homo sapiens
C210S relative specific activity in the extract is nearly the same to that of the wild-type enzyme Homo sapiens
C239S relative specific activity in the extract is nearly the same to that of the wild-type enzyme Homo sapiens
C302S relative specific activity in the extract is nearly the same to that of the wild-type enzyme Homo sapiens
C380S no enzyme activity detected in the extract Homo sapiens
C386S relative specific activity in the extract is nearly the same to that of the wild-type enzyme Homo sapiens
C390S relative specific activity in the extract is nearly the same to that of the wild-type enzyme Homo sapiens
C41S relative specific activity in the extract is nearly the same to that of the wild-type enzyme Homo sapiens
C66S relative specific activity in the extract is nearly the same to that of the wild-type enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis-2-nitrobenzoate 0.1 mM, more than 95% inhibition Homo sapiens
Monoiodoacetic acid 10 mM, more than 95% inhibition Homo sapiens
NaN3 10 mM, 18% inhibition Homo sapiens
NEM 1 mM, more than 95% inhibition Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82000
-
gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-D-mannosamine
-
Homo sapiens N-acetyl-D-glucosamine
-
?

Synonyms

Synonyms Comment Organism
renin binding protein
-
Homo sapiens
RNBP
-
Homo sapiens