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Literature summary for 5.1.3.8 extracted from

  • Tabata, K.; Koizumi, S.; Endo, T.; Ozaki, A.
    Production of N-acetyl-D-neuraminic acid by coupling bacteria expressing N-acetyl-D-glucosamine 2-epimerase and N-acetyl-D-neuraminic acid synthetase (2002), Enzyme Microb. Technol., 30, 327-333.
No PubMed abstract available

Application

Application Comment Organism
synthesis production of N-acetyl-D-neuraminic acid by coupling bacteria expressing N-acetyl-D-glucosamine 2-epimerase and N-acetyl-D-neuraminic acid synthetase. Microbial production of N-acetylneuraminic acid is carried out using Escherichia coli overexpressing N-acylglucosamine 2-epimerase and NeuAc synthetase as enzyme sources. Phosphoenolpyruvate and ATP, required as a substrate or a cofactor of the enzymes, are supplied by the activities of Escherichia coli and Corynebacterium ammoniagenes cells. Starting with 800 mM GlcNAc and 360 mM glucose, NeuAc accumulates at 39.7 mM after 22 h Synechocystis sp.

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Synechocystis sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.76
-
N-acetyl-D-mannosamine
-
Synechocystis sp.
6.94
-
N-acetyl-D-glucosamine
-
Synechocystis sp.

Organism

Organism UniProt Comment Textmining
Synechocystis sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Synechocystis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-D-mannosamine
-
Synechocystis sp. N-acetyl-D-glucosamine
-
r

Synonyms

Synonyms Comment Organism
GlcNAc 2-epimerase
-
Synechocystis sp.
N-Acetylglucosamine 2-epimerase
-
Synechocystis sp.

Cofactor

Cofactor Comment Organism Structure
ATP essential for activity Synechocystis sp.