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Literature summary for 5.1.3.7 extracted from

  • Dong, S.; Chesnokova, O.N.; Turnbough, C.L.; Pritchard, D.G.
    Identification of the UDP-N-acetylglucosamine 4-epimerase involved in exosporium protein glycosylation in Bacillus anthracis (2009), J. Bacteriol., 191, 7094-7101.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.1.3.7

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus anthracis

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37800
-
 predicted by amino acid sequence Bacillus anthracis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bacillus anthracis the gene encodes a bifunctional enzyme with both UDP-GlcNAc 4-epimerase and UDP-Glc 4-epimerase activities and that no other annotated UDP-Glc 4-epimerase gene encodes a UDP-GlcNAc 4-epimerase ?
-
 ?
UDP-N-acetylgalactosamine Bacillus anthracis
-
 UDP-N-acetylglucosamine
-
 r

Organism

Organism UniProt Comment Textmining
Bacillus anthracis Q81JK4
-
-

Purification (Commentary)

Purification (Comment) Organism
by using nickel-nitrilotriacetic acid affinity chromatography and gel filtration Bacillus anthracis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the gene encodes a bifunctional enzyme with both UDP-GlcNAc 4-epimerase and UDP-Glc 4-epimerase activities and that no other annotated UDP-Glc 4-epimerase gene encodes a UDP-GlcNAc 4-epimerase Bacillus anthracis ?
-
 ?
UDP-N-acetylgalactosamine
-
 Bacillus anthracis UDP-N-acetylglucosamine
-
 r

Synonyms

Synonyms Comment Organism
UDP-N-acetylglucosamine 4-epimerase
-
 Bacillus anthracis

Expression

Organism Comment Expression
Bacillus anthracis spores produced by a mutant strain lacking the enzyme still contain normal levels of BclA-attached oligosaccharides, monosaccharide analysis of the oligosaccharides reveals that N-acetylglucosamine had replaced N-acetylgalactosamine, significant levels of gene transcripts are detected only during sporulation, an observation consistent with a role for this gene in exosporium assembly, gene expression during sporulation appears to be biphasic additional information

General Information

General Information Comment Organism
physiological function the epimerase is required to produce N-acetyl-galactosamine for BclA oligosaccharide biosynthesis, a collagen-like glycoprotein, UDP-GlcNAc 4-epimerase encoded by the BAS5304 gene is required for the synthesis of GalNAc in sporulating cells and that GlcNAc can replace GalNAc in the synthesis of BclA oligosaccharides Bacillus anthracis