Literature summary for 5.1.3.7 extracted from

Valiente, E.; Jimenez, N.; Merino, S.; Tomas, J.M.; Amaro, C.
Vibrio vulnificus biotype 2 serovar E gne but not galE is essential for lipopolysaccharide biosynthesis and virulence (2008), Infect. Immun., 76, 1628-1638.

Search for more literature for 5.1.3.7

Application

Application	Comment	Organism
medicine	mutant deficient for gne shows increased surface hydrophobicity, produces deep alterations in the outer membrane architecture, and displays noticeable increases in the sensitivity to microcidal peptides, to eel and human sera, and to phagocytosis/opsonophagocytosis. Significant attenuation of virulence for eels and mice is observed with the mutant, which is correlated with the loss of the O-antigen lipopolysaccharide, while the capsule is maintained	Vibrio vulnificus

Protein Variants

Protein Variants	Comment	Organism
additional information	a gne-deficient mutant completely lacks UDPGalNAc 4-epimerase activity and has UDP-Gal 4-epimerase activity in after growth on D-glucose or D-galactose that is not significantly different from that of the wild-type strain. Mutation increases the surface hydrophobicity, produces deep alterations in the outer membrane architecture, and results in noticeable increases in the sensitivity to microcidal peptides, to eel and human sera, and to phagocytosis/opsonophagocytosis. Significant attenuation of virulence for eels and mice is observed with the mutant	Vibrio vulnificus

Organism

Organism	UniProt	Comment	Textmining
Vibrio vulnificus	Q7MH46	biotype 2 serovar E, isoform Gne	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.0119	-	growth on D-galactose as sole carbon source	Vibrio vulnificus
0.0206	-	growth on D-glucose as sole carbon source	Vibrio vulnificus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-N-acetyl-D-glucosamine	-	Vibrio vulnificus	UDP-N-acetyl-D-galactosamine	-	r

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Release 2023.1 (January 2023)