Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.1.3.7 extracted from

  • Bernatchez, S.; Szymanski, C.M.; Ishiyama, N.; Li, J.; Jarrell, H.C.; Lau, P.C.; Berghuis, A.M.; Young, N.M.; Wakarchuk, W.W.
    A single bifunctional UDP-GlcNAc/Glc 4-epimerase supports the synthesis of three cell surface glycoconjugates in Campylobacter jejuni (2005), J. Biol. Chem., 280, 4792-4802.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Campylobacter jejuni

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.78
-
UDP-D-glucose MalE-Gne fusion protein Campylobacter jejuni
0.784
-
UDP-D-galactose MalE-Gne fusion protein Campylobacter jejuni
1.07
-
UDP-N-acetyl-D-galactosamine MalE-Gne fusion protein Campylobacter jejuni
1.087
-
UDP-N-acetyl-D-glucosamine MalE-Gne fusion protein Campylobacter jejuni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-D-glucose Campylobacter jejuni the enzyme provides Gal and galNAc residues for the synthesis of the cell-surface carbohydrates in Campylobacter jejuni NCTC 11168 UDP-D-galactose
-
r
UDP-N-acetyl-D-glucosamine Campylobacter jejuni the enzyme provides Gal and galNAc residues for the synthesis of the cell-surface carbohydrates in Campylobacter jejuni NCTC 11168 UDP-N-acetyl-D-galactosamine
-
r

Organism

Organism UniProt Comment Textmining
Campylobacter jejuni
-
NCTC 11168
-

Purification (Commentary)

Purification (Comment) Organism
MalE-Gne fusion protein Campylobacter jejuni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-D-glucose
-
Campylobacter jejuni UDP-D-galactose
-
r
UDP-D-glucose the enzyme provides Gal and galNAc residues for the synthesis of the cell-surface carbohydrates in Campylobacter jejuni NCTC 11168 Campylobacter jejuni UDP-D-galactose
-
r
UDP-N-acetyl-D-glucosamine
-
Campylobacter jejuni UDP-N-acetyl-D-galactosamine
-
r
UDP-N-acetyl-D-glucosamine the enzyme provides Gal and galNAc residues for the synthesis of the cell-surface carbohydrates in Campylobacter jejuni NCTC 11168 Campylobacter jejuni UDP-N-acetyl-D-galactosamine
-
r

Synonyms

Synonyms Comment Organism
GNE
-
Campylobacter jejuni
UDP-GlcNAc/Glc 4-epimerase bifunctional enzyme Campylobacter jejuni

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
73.95
-
UDP-D-glucose MalE-Gne fusion protein Campylobacter jejuni
82.3
-
UDP-N-acetyl-D-glucosamine MalE-Gne fusion protein Campylobacter jejuni
247.9
-
UDP-N-acetyl-D-galactosamine MalE-Gne fusion protein Campylobacter jejuni
290.1
-
UDP-D-galactose MalE-Gne fusion protein Campylobacter jejuni