General Stability | Organism |
---|---|
2-mercaptoethanol stabilizes the enzyme increasing its lifetime in the reaction mixture by 50% | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.131 | - |
UDP-N-acetyl-D-galactosamine | pH 8.0, 37°C | Pseudomonas aeruginosa | |
0.137 | - |
UDP-N-acetyl-D-glucosamine | pH 8.0, 37°C | Pseudomonas aeruginosa | |
0.153 | - |
UDP-D-glucose | pH 8.0, 37°C | Pseudomonas aeruginosa | |
0.16 | - |
UDP-D-galactose | pH 8.0, 37°C | Pseudomonas aeruginosa |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | activity is independent of divalent metal | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
gel filtration | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
serotype O6 | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-D-glucose | the achieved reasonable conversion rates the amount of enzyme used is increased 200fold comparted to UDP-N-acetyl-D-glucosamine as substrate. Substrate conversions reach 20% for UDP-Glc and 65% for UDP-Gal | Pseudomonas aeruginosa | UDP-D-galactose | - |
r | |
UDP-N-acetyl-D-glucosamine | equilibrium reaction resulting in a 70:30 ratio of UDP-GlcNAc to UDP-GalNAc, irrespective of the initial substrate | Pseudomonas aeruginosa | UDP-N-acetyl-D-galactosamine | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
WbPP | - |
Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Pseudomonas aeruginosa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 50 | 20% of maximal activity at 10°C and at 50°C | Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.52 | - |
UDP-D-glucose | pH 8.0, 37°C | Pseudomonas aeruginosa | |
3.4 | - |
UDP-D-galactose | pH 8.0, 37°C | Pseudomonas aeruginosa | |
36.9 | - |
UDP-D-galactose | pH 8.0, 37°C | Pseudomonas aeruginosa | |
27660 | - |
UDP-N-acetyl-D-glucosamine | pH 8.0, 37°C | Pseudomonas aeruginosa | |
27700 | - |
UDP-N-acetyl-D-glucosamine | pH 8.0, 37°C | Pseudomonas aeruginosa | |
62280 | - |
UDP-N-acetyl-D-glucosamine | pH 8.0, 37°C | Pseudomonas aeruginosa | |
62300 | - |
UDP-N-acetyl-D-glucosamine | pH 8.0, 37°C | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | - |
Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | tight binding to protein during its synthesis | Pseudomonas aeruginosa | |
NADH | tight binding to protein during its synthesis | Pseudomonas aeruginosa |