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Literature summary for 5.1.3.7 extracted from

  • Creuzenet, C.; Belanger, M.; Wakarchuk, W.W.; Lam, J.S.
    Expression, purification, and biochemical characterization of WbpP, a new UDP-GlcNAc C4 epimerase from Pseudomonas aeruginosa serotype O6 (2000), J. Biol. Chem., 275, 19060-19067.
    View publication on PubMed

General Stability

General Stability Organism
2-mercaptoethanol stabilizes the enzyme increasing its lifetime in the reaction mixture by 50% Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.131
-
UDP-N-acetyl-D-galactosamine pH 8.0, 37°C Pseudomonas aeruginosa
0.137
-
UDP-N-acetyl-D-glucosamine pH 8.0, 37°C Pseudomonas aeruginosa
0.153
-
UDP-D-glucose pH 8.0, 37°C Pseudomonas aeruginosa
0.16
-
UDP-D-galactose pH 8.0, 37°C Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
additional information activity is independent of divalent metal Pseudomonas aeruginosa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
80000
-
gel filtration Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
serotype O6
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-D-glucose the achieved reasonable conversion rates the amount of enzyme used is increased 200fold comparted to UDP-N-acetyl-D-glucosamine as substrate. Substrate conversions reach 20% for UDP-Glc and 65% for UDP-Gal Pseudomonas aeruginosa UDP-D-galactose
-
r
UDP-N-acetyl-D-glucosamine equilibrium reaction resulting in a 70:30 ratio of UDP-GlcNAc to UDP-GalNAc, irrespective of the initial substrate Pseudomonas aeruginosa UDP-N-acetyl-D-galactosamine
-
r

Subunits

Subunits Comment Organism
dimer
-
Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
WbPP
-
Pseudomonas aeruginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Pseudomonas aeruginosa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 50 20% of maximal activity at 10°C and at 50°C Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.52
-
UDP-D-glucose pH 8.0, 37°C Pseudomonas aeruginosa
3.4
-
UDP-D-galactose pH 8.0, 37°C Pseudomonas aeruginosa
36.9
-
UDP-D-galactose pH 8.0, 37°C Pseudomonas aeruginosa
27660
-
UDP-N-acetyl-D-glucosamine pH 8.0, 37°C Pseudomonas aeruginosa
27700
-
UDP-N-acetyl-D-glucosamine pH 8.0, 37°C Pseudomonas aeruginosa
62280
-
UDP-N-acetyl-D-glucosamine pH 8.0, 37°C Pseudomonas aeruginosa
62300
-
UDP-N-acetyl-D-glucosamine pH 8.0, 37°C Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 9
-
Pseudomonas aeruginosa

Cofactor

Cofactor Comment Organism Structure
NAD+ tight binding to protein during its synthesis Pseudomonas aeruginosa
NADH tight binding to protein during its synthesis Pseudomonas aeruginosa