Literature summary for 5.1.3.5 extracted from

Yin, S.; Kong, J.
Transcriptome-guided discovery and functional characterization of two UDP-sugar 4-epimerase families involved in the biosynthesis of anti-tumor polysaccharides in Ornithogalum caudatum (2016), RSC Adv., 6, 37370-37384 .

No PubMed abstract available

Cloned(Commentary)

Cloned (Comment)	Organism
gene UXE1, transcriptome-guided search by transcriptome analysis of Ornithogalum caudatum unigenes, cloning, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of soluble His-tagged N-terminally truncated enzyme in Escherichia coli strain BL21(DE3) by coexpression with chaperones from plasmid pET28atOcUXE1 and chaperone plasmid pGro7, and pET28atOcUXE2 and another chaperone plasmid pKJE7. The recombinant expression in absence of chaperones results in misfolded enzyme in inclusion bodies, expression of full-length His-tagged enzyme is unsuccessful. The truncated recombinant enzyme is catalytically inactive	Albuca bracteata
gene UXE2, transcriptome-guided search by transcriptome analysis of Ornithogalum caudatum unigenes, cloning, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of soluble His-tagged N-terminally truncated enzyme in Escherichia coli strain BL21(DE3) by coexpression with chaperones from plasmid pET28atOcUXE1 and chaperone plasmid pGro7, and pET28atOcUXE2 and another chaperone plasmid pKJE7. The recombinant expression in absence of chaperones results in misfolded enzyme in inclusion bodies, expression of full-length His-tagged enzyme is unsuccessful. The truncated recombinant enzyme is catalytically inactive	Albuca bracteata

Cloned (Comment)

Organism

gene UXE1, transcriptome-guided search by transcriptome analysis of Ornithogalum caudatum unigenes, cloning, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of soluble His-tagged N-terminally truncated enzyme in Escherichia coli strain BL21(DE3) by coexpression with chaperones from plasmid pET28atOcUXE1 and chaperone plasmid pGro7, and pET28atOcUXE2 and another chaperone plasmid pKJE7. The recombinant expression in absence of chaperones results in misfolded enzyme in inclusion bodies, expression of full-length His-tagged enzyme is unsuccessful. The truncated recombinant enzyme is catalytically inactive

Albuca bracteata

gene UXE2, transcriptome-guided search by transcriptome analysis of Ornithogalum caudatum unigenes, cloning, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of soluble His-tagged N-terminally truncated enzyme in Escherichia coli strain BL21(DE3) by coexpression with chaperones from plasmid pET28atOcUXE1 and chaperone plasmid pGro7, and pET28atOcUXE2 and another chaperone plasmid pKJE7. The recombinant expression in absence of chaperones results in misfolded enzyme in inclusion bodies, expression of full-length His-tagged enzyme is unsuccessful. The truncated recombinant enzyme is catalytically inactive

Albuca bracteata

Protein Variants

Protein Variants	Comment	Organism
additional information	generation of truncated enzyme for expression as soluble protein. The truncated recombinant enzyme is catalytically inactive	Albuca bracteata

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	a type I membrane protein	Albuca bracteata	16020	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
UDP-L-arabinose	Albuca bracteata	-	UDP-D-xylose	-	r

Organism

Organism	UniProt	Comment	Textmining
Albuca bracteata	A0A168GPQ3	UXE1; i.e. Albuca bracteata or Ornithogalum longebracteatum	-
Albuca bracteata	A0A168GPR3	UXE2; i.e. Albuca bracteata or Ornithogalum longebracteatum	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged truncated inactive enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography	Albuca bracteata

Source Tissue

Source Tissue	Comment	Organism	Textmining
bulb	sterile	Albuca bracteata	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	enzyme UXE, has strict substrate specificity and only catalyzes the interconversion of UDP-D-Xyl and UDP-L-Ara	Albuca bracteata	?	-	?
UDP-L-arabinose	-	Albuca bracteata	UDP-D-xylose	-	r

Subunits

Subunits	Comment	Organism
?	x * 45400, about, sequence calculation	Albuca bracteata
?	x * 46200, about, sequence calculation	Albuca bracteata

Synonyms

Synonyms	Comment	Organism
UDP-xylose 4-epimerase	-	Albuca bracteata
Uxe	-	Albuca bracteata
UXE1	-	Albuca bracteata
UXE2	-	Albuca bracteata

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Albuca bracteata

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	assay at	Albuca bracteata

pI Value

Organism	Comment	pI Value Maximum	pI Value
Albuca bracteata	sequence calculation	-	6.93
Albuca bracteata	sequence calculation	-	9.05

General Information

General Information	Comment	Organism
physiological function	the enzyme is involved in the biosynthesis of anti-tumor polysaccharides in Ornithogalum caudatum. Enzyme UXE also plays a key role in carbohydrate partitioning, plant growth and development. UXE is important in the composition of the sugar nucleotide pools	Albuca bracteata