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Literature summary for 5.1.3.5 extracted from

  • Kotake, T.; Takata, R.; Verma, R.; Takaba, M.; Yamaguchi, D.; Orita, T.; Kaneko, S.; Matsuoka, K.; Koyama, T.; Reiter, W.D.; Tsumuraya, Y.
    Bifunctional cytosolic UDP-glucose 4-epimerases catalyse the interconversion between UDP-D-xylose and UDP-L-arabinose in plants (2009), Biochem. J., 424, 169-177.
    View publication on PubMed
Search for more literature for 5.1.3.5

Cloned(Commentary)

Cloned (Comment) Organism
recombinant protein AtUGE1-5 is expressed in Escherichia coli with C-terminal His-tag Arabidopsis thaliana
recombinant protein is expressed in Escherichia coli Pisum sativum
recombinant protein is expressed in Escherichia coli with C-terminal His-tag Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 UDP-D-xylose 50 mM Tris-HCl buffer (pH 8.6), 0.1 mM NAD+, 1 mM UDP-sugar and enzyme Pisum sativum
0.016
-
 UDP-L-arabinose 50 mM Tris-HCl buffer (pH 8.6), 0.1 mM NAD+, 1 mM UDP-sugar and enzyme Pisum sativum
0.16
-
 UDP-D-xylose recombinant Arabidopsis thaliana enzyme, UDP-Xyl 4-epimerase activity is monitored by an end-point assay containing 0.1-5.0 mM UDP-xylose, 100 mM glycine/NaOH (pH 8.6) Arabidopsis thaliana
0.32
-
 UDP-D-xylose recominant Arabidopsis thaliana enzyme UGE3, UDP-Xyl 4-epimerase activity is monitored by an end-point assay containing 0.1-5.0 mM UDP-xylose, 100 mM glycine/NaOH (pH 8.6) Arabidopsis thaliana
0.34
-
 UDP-D-xylose recombinant Arabidopsis thaliana enzyme UGE2, UDP-Xyl 4-epimerase activity is monitored by an end-point assay containing 0.1-5.0 mM UDP-xylose, 100 mM glycine/NaOH (pH 8.6) Arabidopsis thaliana
0.42
-
 UDP-D-xylose recombinant Arabidopsis thaliana enzyme UGE4, UDP-Xyl 4-epimerase activity is monitored by an end-point assay containing 0.1-5.0 mM UDP-xylose, 100 mM glycine/NaOH (pH 8.6) Arabidopsis thaliana
0.43
-
 UDP-D-xylose recombinant Arabidopsis thaliana enzyme UGE1, UDP-Xyl 4-epimerase activity is monitored by an end-point assay containing 0.1-5.0 mM UDP-xylose, 100 mM glycine/NaOH (pH 8.6) Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Arabidopsis thaliana 5829
-
cytosol
-
 Pisum sativum 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Al3+ activity of enzyme towards UDP-xylose is 15.5 U/mg protein at a metal concentration of 1 mM Pisum sativum
Ba2+ activity of enzyme towards UDP-xylose is 23.0 U/mg protein at a metal concentration of 1 mM Pisum sativum
Ca2+ activity of enzyme towards UDP-xylose is 20.2 U/mg protein at a metal concentration of 1 mM Pisum sativum
Co2+ activity of enzyme towards UDP-xylose is 18.6 U/mg protein at a metal concentration of 1 mM Pisum sativum
Cu2+ activity of enzyme towards UDP-xylose is 13.9 U/mg protein at a metal concentration of 1 mM Pisum sativum
Fe3+ activity of enzyme towards UDP-xylose is 12.1 U/mg protein at a metal concentration of 1 mM Pisum sativum
Hg2+ 1 mM Hg2+ abolishes enzyme function entirely Pisum sativum
Li+ activity of enzyme towards UDP-xylose is 18.1 U/mg protein at a metal concentration of 1 mM Pisum sativum
Mg2+ activity of enzyme towards UDP-xylose is 21.3 U/mg protein at a metal concentration of 1 mM Pisum sativum
Mn2+ activity of enzyme towards UDP-xylose is 15.5 U/mg protein at a metal concentration of 1 mM Pisum sativum
Zn2+ activity of enzyme towards UDP-xylose is 10.2 U/mg protein at a metal concentration of 1 mM Pisum sativum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
 native Pisum sativum enzyme determined by SDS-PAGE Pisum sativum
41000
-
 recombinant enzyme after purification and thrombin digestion Pisum sativum
56000
-
 recombinant enzyme after purification on a chelating column Pisum sativum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-D-xylose Arabidopsis thaliana
-
 UDP-L-arabinose
-
 r
UDP-D-xylose Pisum sativum
-
 UDP-L-arabinose
-
 r

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q42605
-
-
Arabidopsis thaliana Q8LDN8
-
-
Arabidopsis thaliana Q9C7W7
-
-
Arabidopsis thaliana Q9SN58
-
-
Arabidopsis thaliana Q9T0A7
-
-
Pisum sativum B0M3E8
-
-

Purification (Commentary)

Purification (Comment) Organism
purification of the native enzyme from pea sprouts by ion exchange chromatography and gel filtration, further purification on a hydroxyapatite column, purification of recombinant enzymes by ion exchange chromatography Pisum sativum
purification of the recombinant Arabidopsis enzyme by Ni-NTA affinity chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
sprout
-
 Arabidopsis thaliana
-
sprout
-
 Pisum sativum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.7
-
 native protein, substrate is UDP-arabinose Pisum sativum
6.7
-
 native protein, substrate is UDP-xylose Pisum sativum
16.7
-
 specific activity of recombinant enzyme in cell lysate after expression in Escherichia coli Pisum sativum
21.1
-
 recombinant Pisum protein, substrate is UDP-arabinose Pisum sativum
22.4
-
 recombinant Pisum protein, substrate is UDP-xylose Pisum sativum
38.9
-
 specific activity of recombinant enzyme after purification by a DEAE-sepharose FF column Pisum sativum
49
-
 specific activity of recombinant enzyme after purification by a Ni-sepharose colum Pisum sativum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-D-xylose
-
 Arabidopsis thaliana UDP-L-arabinose
-
 r
UDP-D-xylose
-
 Pisum sativum UDP-L-arabinose
-
 r

Synonyms

Synonyms Comment Organism
AtUGE1
-
 Arabidopsis thaliana
AtUGE2
-
 Arabidopsis thaliana
AtUGE3
-
 Arabidopsis thaliana
AtUGE4
-
 Arabidopsis thaliana
AtUGE5
-
 Arabidopsis thaliana
PsUGE1
-
 Pisum sativum
UDP-Xyl 4-epimerase
-
 Arabidopsis thaliana
UDP-Xyl 4-epimerase
-
 Pisum sativum
UDP-xylose 4-epimerase
-
 Arabidopsis thaliana
UDP-xylose 4-epimerase
-
 Pisum sativum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
15
-
 maximum activity of recombinant enzyme Pisum sativum
25
-
 assay at Arabidopsis thaliana
30
-
 maximum activity of native enzyme Pisum sativum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 recombinant Pisum enzyme loses both activities completely at temperatures higher than 40°C Pisum sativum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
 UDP-D-xylose recombinant Arabidopsis thaliana enzyme Arabidopsis thaliana
0.4
-
 UDP-D-xylose recombinant Arabidopsis thaliana enzyme Arabidopsis thaliana
0.5
-
 UDP-D-xylose recombinant Arabidopsis thaliana enzyme Arabidopsis thaliana
13
-
 UDP-D-xylose recominant Arabidopsis thaliana enzyme UGE3 Arabidopsis thaliana
19
-
 UDP-D-xylose recombinant Arabidopsis thaliana enzyme UGE1 Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.6
-
 assay at Arabidopsis thaliana
8.6
-
 assay at Pisum sativum

pH Range

pH Minimum pH Maximum Comment Organism
8.5 9 maximum activity of recombinant enzyme Pisum sativum

General Information

General Information Comment Organism
physiological function bifunctional cytosolic UDP-glucose 4-epimerase catalyses the interconversion between UDP-D-xylose and UDP-L-arabinose in plants Arabidopsis thaliana
physiological function bifunctional cytosolic UDP-glucose 4-epimerase catalyses the interconversion between UDP-D-xylose and UDP-L-arabinose in plants Pisum sativum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.5
-
 UDP-D-xylose recombinant Arabidopsis thaliana enzyme UGE4, UDP-Xyl 4-epimerase activity is monitored by an end-point assay containing 0.1-5.0 mM UDP-xylose, 100 mM glycine/NaOH (pH 8.6) Arabidopsis thaliana
1.2
-
 UDP-D-xylose recombinant Arabidopsis thaliana enzyme, UDP-Xyl 4-epimerase activity is monitored by an end-point assay containing 0.1-5.0 mM UDP-xylose, 100 mM glycine/NaOH (pH 8.6) Arabidopsis thaliana
3.1
-
 UDP-D-xylose recominant Arabidopsis thaliana enzyme UGE5, UDP-Xyl 4-epimerase activity is monitored by an end-point assay containing 0.1-5.0 mM UDP-xylose, 100 mM glycine/NaOH (pH 8.6) Arabidopsis thaliana
40
-
 UDP-D-xylose recominant Arabidopsis thaliana enzyme UGE3, UDP-Xyl 4-epimerase activity is monitored by an end-point assay containing 0.1-5.0 mM UDP-xylose, 100 mM glycine/NaOH (pH 8.6) Arabidopsis thaliana
45
-
 UDP-D-xylose recombinant Arabidopsis thaliana enzyme UGE1, UDP-Xyl 4-epimerase activity is monitored by an end-point assay containing 0.1-5.0 mM UDP-xylose, 100 mM glycine/NaOH (pH 8.6) Arabidopsis thaliana