Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.1.3.35 extracted from

  • Zhang, C.S.; Podeschwa, M.; Altenbach, H.J.; Piepersberg, W.; Wehmeier, U.F.
    The acarbose-biosynthetic enzyme AcbO from Actinoplanes sp. SE 50/110 is a 2-epi-5-epi-valiolone-7-phosphate 2-epimerase (2003), FEBS Lett., 540, 47-52.
    View publication on PubMed
Search for more literature for 5.1.3.35

Cloned(Commentary)

Cloned (Comment) Organism
gene acbO, encoded in the acbKMLNOC operon, overexpression of N-terminally His-tagged enzyme in Streptomyces lividans strain TK64 under the control of an inducible promoter (ptipA) Actinoplanes sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29500
-
 x * 29500, recombinant His-tagged enzyme, SDS-PAGE, x * 30616, sequence calculation Actinoplanes sp.
30616
-
 x * 29500, recombinant His-tagged enzyme, SDS-PAGE, x * 30616, sequence calculation Actinoplanes sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-epi-5-epi-valiolone 7-phosphate Actinoplanes sp.
-
 5-epi-valiolone 7-phosphate
-
 r

Organism

Organism UniProt Comment Textmining
Actinoplanes sp. Q8RMD1 gene acbO
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Streptomyces lividans strain TK64 by nickel affinity chromatgraphy Actinoplanes sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-epi-5-epi-valiolone 7-phosphate
-
 Actinoplanes sp. 5-epi-valiolone 7-phosphate
-
 r
2-epi-5-epi-valiolone 7-phosphate 2-epimerisation Actinoplanes sp. 5-epi-valiolone 7-phosphate chemical structure of the purified reaction product is proven by NMR spectroscopy, forms a and b via ring flip, overview r

Subunits

Subunits Comment Organism
? x * 29500, recombinant His-tagged enzyme, SDS-PAGE, x * 30616, sequence calculation Actinoplanes sp.

Synonyms

Synonyms Comment Organism
AcbO
-
 Actinoplanes sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Actinoplanes sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Actinoplanes sp.

Cofactor

Cofactor Comment Organism Structure
additional information the purfied recombinant His-tagged AcbO protein is actively converting the product of the AcbM-catalysed reaction in absence of any other cofactor or coenzyme Actinoplanes sp.

General Information

General Information Comment Organism
metabolism the enzyme is involved in the acarbose biosynthesis, pathway overview. The C7-cyclitol 2-epi-5-epi-valiolone is the first precursor of the cyclitol moiety of the alpha-glucosidase inhibitor acarbose in Actinoplanes sp. SE50. The 2-epi-5-epi-valiolone becomes phosphorylated at C7 by the ATP-dependent kinase AcbM prior to the next modifications Actinoplanes sp.