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Literature summary for 5.1.3.30 extracted from
- High-level intra- and extra-cellular production of D-psicose 3-epimerase via a modified xylose-inducible expression system in Bacillus subtilis (2016), J. Ind. Microbiol. Biotechnol., 43, 1577-1591 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene rdpe, recombinant constitutive enzyme expression in Bacillus subtilis strains 1A751-SR, 1A751-GR, and 1A751S1-XR (from strain 1A751), in which two extracellular proteases (nprE, aprE) are deleted, Bacillus subtilis is transformed using the Paris method, screening and characterization of different inducible promoters, xylose-inducible expression system, method evaluation, detailed overview | Ruminococcus sp. 5_1_39BFAA |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | high-level intra- and extra-cellular production of D-psicose 3-epimerase via a modified xylose-inducible expression system in Bacillus subtilis, fed-batch fermentation in 7.5 l fermentor, overview | Ruminococcus sp. 5_1_39BFAA |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-psicose | Ruminococcus sp. 5_1_39BFAA | - |
D-fructose | - |
r |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ruminococcus sp. 5_1_39BFAA | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-psicose | - |
Ruminococcus sp. 5_1_39BFAA | D-fructose | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DPEase | - |
Ruminococcus sp. 5_1_39BFAA |
| RDPE | - |
Ruminococcus sp. 5_1_39BFAA |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
assay at | Ruminococcus sp. 5_1_39BFAA |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Ruminococcus sp. 5_1_39BFAA |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Ruminococcus sp. 5_1_39BFAA | xylose induces the expression of recombinant RDPE | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | D-psicose 3-epimerase (DPEase) converts D-fructose into d-psicose which exists in nature in limited quantities and has key physiological functions | Ruminococcus sp. 5_1_39BFAA |
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Release 2023.1 (January 2023)
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