Literature summary for 5.1.3.30 extracted from

Chen, J.; Jin, Z.; Gai, Y.; Sun, J.; Zhang, D.
A food-grade expression system for D-psicose 3-epimerase production in Bacillus subtilis using an alanine racemase-encoding selection marker (2017), Bioresour. Bioproc., 4, 9 .

Cloned(Commentary)

Cloned (Comment)	Organism
gene rdpe, recombinant expression in and secretion from Bacillus subtilis alanine racemase knockout (deletion of gene dal using Cre/lox system) strains 1A751D2R and 1A751D2C, construction of food-grade expression plasmids with auxotrophic marker, the food-grade plasmids pMA5-DAL-RDPE and pMA5-DAL are transformed into the food-grade host strain 1A751D2 with the deficiency of alanine racemase gene to generate the recombinant strain 1A751D2R and 1A751D2C, respectively. The extracellular activity of enzyme RDPE is gradually increased with the fermentation process, and the highest activity reaches 46 U/ml at 72 h, fed-batch fermentation, method evaluation, overview	Ruminococcus sp. 5_1_39BFAA

Cloned (Comment)

Organism

gene rdpe, recombinant expression in and secretion from Bacillus subtilis alanine racemase knockout (deletion of gene dal using Cre/lox system) strains 1A751D2R and 1A751D2C, construction of food-grade expression plasmids with auxotrophic marker, the food-grade plasmids pMA5-DAL-RDPE and pMA5-DAL are transformed into the food-grade host strain 1A751D2 with the deficiency of alanine racemase gene to generate the recombinant strain 1A751D2R and 1A751D2C, respectively. The extracellular activity of enzyme RDPE is gradually increased with the fermentation process, and the highest activity reaches 46 U/ml at 72 h, fed-batch fermentation, method evaluation, overview

Ruminococcus sp. 5_1_39BFAA

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-psicose	Ruminococcus sp. 5_1_39BFAA	-	D-fructose	-	r

Organism

Organism	UniProt	Comment	Textmining
Ruminococcus sp. 5_1_39BFAA	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-psicose	-	Ruminococcus sp. 5_1_39BFAA	D-fructose	-	r

Synonyms

Synonyms	Comment	Organism
RDPE	-	Ruminococcus sp. 5_1_39BFAA

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Release 2023.1 (January 2023)