Cloned (Comment) | Organism |
---|---|
gene rdpe, recombinant expression in and secretion from Bacillus subtilis alanine racemase knockout (deletion of gene dal using Cre/lox system) strains 1A751D2R and 1A751D2C, construction of food-grade expression plasmids with auxotrophic marker, the food-grade plasmids pMA5-DAL-RDPE and pMA5-DAL are transformed into the food-grade host strain 1A751D2 with the deficiency of alanine racemase gene to generate the recombinant strain 1A751D2R and 1A751D2C, respectively. The extracellular activity of enzyme RDPE is gradually increased with the fermentation process, and the highest activity reaches 46 U/ml at 72 h, fed-batch fermentation, method evaluation, overview | Ruminococcus sp. 5_1_39BFAA |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-psicose | Ruminococcus sp. 5_1_39BFAA | - |
D-fructose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ruminococcus sp. 5_1_39BFAA | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-psicose | - |
Ruminococcus sp. 5_1_39BFAA | D-fructose | - |
r |
Synonyms | Comment | Organism |
---|---|---|
RDPE | - |
Ruminococcus sp. 5_1_39BFAA |