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Literature summary for 5.1.3.30 extracted from
- Characterization of a novel metal-dependent D-psicose 3-epimerase from Clostridium scindens 35704 (2013), PLoS One, 8, e62987.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | [Clostridium] scindens |
General Stability
| General Stability | Organism |
|---|---|
| Mn2+ improves the structural stability during both heat- and urea-induced unfolding | [Clostridium] scindens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | 8.3 | D-psicose | pH 7.5, 60°C | [Clostridium] scindens |  |
| 40.1 | - |
D-fructose | pH 7.5, 60°C | [Clostridium] scindens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | the enzyme is strictly metal-dependent and requires Mn2+ as optimum cofactor for activity | [Clostridium] scindens | |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| urea | midtransition concentration: 2.68 M | [Clostridium] scindens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| [Clostridium] scindens | B0NGC5 | - |
- |
| [Clostridium] scindens ATCC 35704 | B0NGC5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
[Clostridium] scindens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose | the catalytic efficiency for D-psicose is 7.4fold higher than that for D-tagatose. The equilibrium ratio between D-psicose and D-fructose is 28:72. The catalytic efficiency for D-psicose is 258fold higher than that for D-tagatose | [Clostridium] scindens | D-psicose | - |
r | |
| D-fructose | the catalytic efficiency for D-psicose is 7.4fold higher than that for D-tagatose. The equilibrium ratio between D-psicose and D-fructose is 28:72. The catalytic efficiency for D-psicose is 258fold higher than that for D-tagatose | [Clostridium] scindens ATCC 35704 | D-psicose | - |
r | |
| D-psicose | the catalytic efficiency for D-psicose is 7.4fold higher than that for D-tagatose. The equilibrium ratio between D-psicose and D-fructose is 28:72. The catalytic efficiency for D-psicose is 258fold higher than that for D-tagatose | [Clostridium] scindens | D-fructose | - |
r | |
| D-psicose | the catalytic efficiency for D-psicose is 7.4fold higher than that for D-tagatose. The equilibrium ratio between D-psicose and D-fructose is 28:72. The catalytic efficiency for D-psicose is 258fold higher than that for D-tagatose | [Clostridium] scindens ATCC 35704 | D-fructose | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CLOSCI_02528 | locus name | [Clostridium] scindens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
[Clostridium] scindens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 75 | 35°C: about 45% of maximal activity, 75°C: about 55% of maximal activity | [Clostridium] scindens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
half-life: 108 min | [Clostridium] scindens |
| 55 | - |
1 h, about 40% loss of activity | [Clostridium] scindens |
| 60 | - |
1 h, about 55% loss of activity | [Clostridium] scindens |
| 64.4 | - |
apparent melting temperature | [Clostridium] scindens |
| 65 | - |
1 h, about 80% loss of activity | [Clostridium] scindens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 350 | - |
D-fructose | pH 7.5, 60°C | [Clostridium] scindens | |
| 1827 | - |
D-psicose | pH 7.5, 60°C | [Clostridium] scindens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
[Clostridium] scindens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9 | pH 5.5: about 66% of maximal activity, pH 9.0: about 65% of maximal activity | [Clostridium] scindens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
4°C, 2 h, about 30% loss of activity | [Clostridium] scindens |
| 6 | - |
4°C, 2 h, about 15% loss of activity | [Clostridium] scindens |
| 6.5 | - |
4°C, 2 h, about 15% loss of activity | [Clostridium] scindens |
| 7 | - |
4°C, 2 h, about 5% loss of activity | [Clostridium] scindens |
| 7.5 | - |
4°C, 2 h, less than 5% loss of activity | [Clostridium] scindens |
| 8 | - |
4°C, 2 h, about 10% loss of activity | [Clostridium] scindens |
| 8.5 | - |
4°C, 2 h, about 10% loss of activity | [Clostridium] scindens |
| 9 | - |
4°C, 2 h, about 20% loss of activity | [Clostridium] scindens |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8.72 | - |
D-fructose | pH 7.5, 60°C | [Clostridium] scindens | |
| 64.5 | - |
D-psicose | pH 7.5, 60°C | [Clostridium] scindens | |
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