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Literature summary for 5.1.3.30 extracted from
- Cloning, expression, and characterization of a D-psicose 3-epimerase from Clostridium cellulolyticum H10 (2011), J. Agric. Food Chem., 59, 7785-7792.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Ruminiclostridium cellulolyticum |
General Stability
| General Stability | Organism |
|---|---|
| the enzyme is extremely thermostable in the presence of Co2+ but readily inactivated without metal ion | Ruminiclostridium cellulolyticum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 17.4 | - |
D-psicose | pH 8.0, 55°C | Ruminiclostridium cellulolyticum |  |
| 53.5 | - |
D-fructose | pH 8.0, 55°C | Ruminiclostridium cellulolyticum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | the enzyme is strictly metal-dependent and shows a maximal activity in the presence of Co2+ | Ruminiclostridium cellulolyticum | |
| Fe2+ | the enzyme is strictly metal-dependent and shows a maximal activity in the presence of Co2+. When Co2+ is replaced with Fe2+ the enzyme activity is reduced to 67% of that in presence of Co2+ | Ruminiclostridium cellulolyticum | |
| Mg2+ | the enzyme is strictly metal-dependent and shows a maximal activity in the presence of Co2+. When Co2+ is replaced with Mg2+ the enzyme activity is reduced to 38% of that in presence of Co2+ | Ruminiclostridium cellulolyticum | |
| Mn2+ | the enzyme is strictly metal-dependent and shows a maximal activity in the presence of Co2+. When Co2+ is replaced with Mn2+ the enzyme activity is reduced to 92% of that in presence of Co2+ | Ruminiclostridium cellulolyticum | |
| Ni2+ | the enzyme is strictly metal-dependent and shows a maximal activity in the presence of Co2+. When Co2+ is replaced with Ni2+ the enzyme activity is reduced to 65% of that in presence of Co2+ | Ruminiclostridium cellulolyticum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
4 * 32000, SDS-PAGE | Ruminiclostridium cellulolyticum |
| 130000 | - |
gel filtration | Ruminiclostridium cellulolyticum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ruminiclostridium cellulolyticum | B8I944 | - |
- |
| Ruminiclostridium cellulolyticum ATCC 35319 | B8I944 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| nickel-affinity chromatography | Ruminiclostridium cellulolyticum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose | the enzyme carries out the epimerization of D-fructose to D-psicose with a conversion yield of 32% under optimal conditions | Ruminiclostridium cellulolyticum | D-psicose | - |
r | |
| D-fructose | the enzyme carries out the epimerization of D-fructose to D-psicose with a conversion yield of 32% under optimal conditions | Ruminiclostridium cellulolyticum ATCC 35319 | D-psicose | - |
r | |
| D-psicose | the enzyme carries out the epimerization of D-fructose to D-psicose with a conversion yield of 32% under optimal conditions | Ruminiclostridium cellulolyticum | D-fructose | - |
r | |
| D-psicose | the enzyme carries out the epimerization of D-fructose to D-psicose with a conversion yield of 32% under optimal conditions | Ruminiclostridium cellulolyticum ATCC 35319 | D-fructose | - |
r | |
| additional information | the relative activity with D-tagatose is 4.9% of the activity with D-psicose, the catalytic efficiency for D-tagatose is 249fold lower than the catalytic efficiency for D-fructose, the relative activity with D-sorbose is 0.9% of the activity wth D-psicose. No activity with D-fructose-6-phosphate or D-ribulose-5-phosphate | Ruminiclostridium cellulolyticum | ? | - |
? | |
| additional information | the relative activity with D-tagatose is 4.9% of the activity with D-psicose, the catalytic efficiency for D-tagatose is 249fold lower than the catalytic efficiency for D-fructose, the relative activity with D-sorbose is 0.9% of the activity wth D-psicose. No activity with D-fructose-6-phosphate or D-ribulose-5-phosphate | Ruminiclostridium cellulolyticum ATCC 35319 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 32000, SDS-PAGE | Ruminiclostridium cellulolyticum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ACL75304 protein | - |
Ruminiclostridium cellulolyticum |
| Ccel_0941 | - |
Ruminiclostridium cellulolyticum |
| DPEase | ambiguous | Ruminiclostridium cellulolyticum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Ruminiclostridium cellulolyticum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 70 | 35°C: about 50% of maximal activity, 70°C: about 65% of maximal activity | Ruminiclostridium cellulolyticum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
the half-lives for the enzyme are 9.5 h and 24 min when incubated with and without Co2+ (0.1 mM) | Ruminiclostridium cellulolyticum |
| 60 | - |
the half-lives for the enzyme are 6.8 h and 10 min when incubated with and without Co2+ (0.1 mM) | Ruminiclostridium cellulolyticum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 54.1 | - |
D-psicose | pH 8.0, 55°C | Ruminiclostridium cellulolyticum | |
| 55.9 | - |
D-fructose | pH 8.0, 55°C | Ruminiclostridium cellulolyticum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Ruminiclostridium cellulolyticum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | 6.0: about 60% of maximal activity, pH 9.0: about 70% of maximal activity | Ruminiclostridium cellulolyticum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
4°C, 2 h, 15% loss of activity | Ruminiclostridium cellulolyticum |
| 6.5 | - |
4°C, 2 h, 10% loss of activity | Ruminiclostridium cellulolyticum |
| 7 | - |
4°C, 2 h, 5% loss of activity | Ruminiclostridium cellulolyticum |
| 7.5 | 8 | 4°C, 2 h, stable | Ruminiclostridium cellulolyticum |
| 8.5 | 9 | 4°C, 2 h, 10% loss of activity | Ruminiclostridium cellulolyticum |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
D-fructose | pH 8.0, 55°C | Ruminiclostridium cellulolyticum | |
| 3.1 | - |
D-psicose | pH 8.0, 55°C | Ruminiclostridium cellulolyticum | |
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