Cloned (Comment) | Organism |
---|---|
gene galM, DNA and amino acid sequence determination and analysis, sequence comparisons, transcription of the galETK operon is initiated from a single promoter, recombinant expression of His6-tagged enzyme in Escherichia coli strain MG1655 | Yersinia pestis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | galactose mutarotase (galM) gene is inactivated by a single-base-pair deletion, activity of the galM gene can be restored by different single-base-pair insertions | Yersinia pestis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-galactose | Yersinia pestis | - |
beta-D-galactose | - |
r | |
alpha-D-galactose | Yersinia pestis KIM D27 | - |
beta-D-galactose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Yersinia pestis | - |
gene galM | - |
Yersinia pestis KIM D27 | - |
gene galM | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain MG1655 | Yersinia pestis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-galactose | - |
Yersinia pestis | beta-D-galactose | - |
r | |
alpha-D-galactose | - |
Yersinia pestis KIM D27 | beta-D-galactose | - |
r |
Synonyms | Comment | Organism |
---|---|---|
galactose mutarotase | - |
Yersinia pestis |
GalM | - |
Yersinia pestis |
General Information | Comment | Organism |
---|---|---|
evolution | the galM pseudogene has not been deleted during the evolution of Yersinia pestis to become a vector-transmitted systemic pathogen because its reactivation may be beneficial in certain environments. The galactose mutarotase (galM) gene of Yersinia pestis can be converted to a pseudogene by a single-base-pair deletion, overview | Yersinia pestis |
metabolism | GalM function and regulation in the galactose network, overview | Yersinia pestis |
physiological function | the two anomers of D-galactose are used for different purposes, alpha-D-galactose as a carbon source and beta-D-galactose for induction of UDP-galactose synthesis for biosynthetic glycosylation | Yersinia pestis |