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Literature summary for 5.1.3.3 extracted from

  • Thoden, J.B.; Kim, J.; Raushel, F.M.; Holden, H.M.
    The catalytic mechanism of galactose mutarotase (2003), Protein Sci., 12, 1051-1059.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.1.3.3

Crystallization (Commentary)

Crystallization (Comment) Organism
mutants H96N, H170N, D243N, D243A, E304Q, E304A, complexed with either D-glucose or D-galactose Lactococcus lactis

Protein Variants

Protein Variants Comment Organism
D243A drastic decrease in activity Lactococcus lactis
D243N decrease in kcat-value, increase in Km-value Lactococcus lactis
E304A no enzymic activity Lactococcus lactis
E304Q no enzymic activity Lactococcus lactis
H170N no enzymic activity Lactococcus lactis
H96N large decrease in kcat-value Lactococcus lactis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
19
-
 alpha-L-arabinose mutant D243A, pH 7.6, 25°C Lactococcus lactis
20
-
 alpha-D-galactose wild-type, pH 7.6, 25°C Lactococcus lactis
21
-
 alpha-D-xylose wild-type, pH 7.6, 25°C Lactococcus lactis
32
-
 alpha-L-arabinose wild-type, pH 7.6, 25°C Lactococcus lactis
33
-
 alpha-D-xylose mutant D243N, pH 7.6, 25°C Lactococcus lactis
34
-
 alpha-D-glucose wild-type, pH 7.6, 25°C Lactococcus lactis
38
-
 alpha-D-galactose mutant D243N, pH 7.6, 25°C Lactococcus lactis
46
-
 alpha-D-glucose mutant H96N, pH 7.6, 25°C Lactococcus lactis
52
-
 alpha-D-galactose mutant D243A, pH 7.6, 25°C Lactococcus lactis
58
-
 alpha-L-arabinose mutant H96N, pH 7.6, 25°C Lactococcus lactis
86
-
 alpha-D-xylose mutant H96N, pH 7.6, 25°C Lactococcus lactis
120
-
 alpha-D-glucose mutant D243N, pH 7.6, 25°C Lactococcus lactis
170
-
 alpha-L-arabinose mutant D243N, pH 7.6, 25°C Lactococcus lactis

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
-
-

Reaction

Reaction Comment Organism Reaction ID
alpha-D-glucose = beta-D-glucose residues E304 and H170 are critical for catalysis and H96 and D243 are important for substrate positioning Lactococcus lactis
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-galactose
-
 Lactococcus lactis beta-D-galactose
-
 ?
alpha-D-glucose
-
 Lactococcus lactis beta-D-glucose
-
 ?
alpha-D-xylose
-
 Lactococcus lactis beta-D-xylose
-
 ?
alpha-L-arabinose
-
 Lactococcus lactis beta-L-arabinose
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
 alpha-D-xylose mutant D243A, pH 7.6, 25°C Lactococcus lactis
2.1
-
 alpha-D-glucose mutant D243A, pH 7.6, 25°C Lactococcus lactis
9
-
 alpha-L-arabinose mutant D243A, pH 7.6, 25°C Lactococcus lactis
17
-
 alpha-D-glucose mutant H96N, pH 7.6, 25°C Lactococcus lactis
50
-
 alpha-D-galactose mutant D243A, pH 7.6, 25°C Lactococcus lactis
62
-
 alpha-L-arabinose mutant H96N, pH 7.6, 25°C Lactococcus lactis
92
-
 alpha-D-xylose mutant H96N, pH 7.6, 25°C Lactococcus lactis
140
-
 alpha-D-xylose mutant D243N, pH 7.6, 25°C Lactococcus lactis
170
-
 alpha-D-galactose mutant H96N, pH 7.6, 25°C Lactococcus lactis
250
-
 alpha-D-glucose mutant D243N, pH 7.6, 25°C Lactococcus lactis
410
-
 alpha-D-xylose wild-type, pH 7.6, 25°C Lactococcus lactis
430
-
 alpha-D-glucose wild-type, pH 7.6, 25°C Lactococcus lactis
1500
-
 alpha-L-arabinose mutant D243N, pH 7.6, 25°C Lactococcus lactis
2400
-
 alpha-D-galactose mutant D243N, pH 7.6, 25°C Lactococcus lactis
3700
-
 alpha-D-galactose wild-type, pH 7.6, 25°C Lactococcus lactis
5500
-
 alpha-L-arabinose wild-type, pH 7.6, 25°C Lactococcus lactis