Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
vapor diffusion method, 1.5 A resolution | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
E209A | the mutant of YjhT is severely impaired in its ability to enhance the mutarotation rate above the spontaneous rate. Circular dichroism spectrum of the mutant is indistinguishable from that of the wild-type protein, suggesting that the phenotype is not because of misfolding of YjhT | Escherichia coli |
E325A | mutation has no effect on the activity | Escherichia coli |
H278A | mutation has no effect on the activity | Escherichia coli |
K11A | mutation has no effect on the activity | Escherichia coli |
K283A | mutation has no effect on the activity | Escherichia coli |
R215A | mutant shows a decreased but observable activity. Circular dichroism spectrum of the mutant is indistinguishable from that of the wild-type protein, suggesting that the phenotype is not because of misfolding of YjhT | Escherichia coli |
Y309A | mutation has no effect on the activity | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Escherichia coli | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38685 | - |
2 * 38685, electrospray mass spectrometry | Escherichia coli |
73900 | - |
equilibrium sedimentation | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-alpha-neuraminate | Escherichia coli | probably facilitates sialidase-negative bacteria to compete sucessfully for limited amounts of extracellular Neu5Ac, which is likely taken up in he beta-anomer. In addition, the rapid removal of sialic acid from solution might be advantageous to the bacterium to damp down host responses | N-acetyl-beta-neuraminate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P39371 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-acetyl-alpha-neuraminate | probably facilitates sialidase-negative bacteria to compete sucessfully for limited amounts of extracellular Neu5Ac, which is likely taken up in he beta-anomer. In addition, the rapid removal of sialic acid from solution might be advantageous to the bacterium to damp down host responses | Escherichia coli | N-acetyl-beta-neuraminate | - |
? | |
N-acetyl-alpha-neuraminate | the enzyme converts alpha-N-acetylneuranimic acid to the beta-anomer, accelerating the equilibrium between the alpha- and beta-anomers | Escherichia coli | N-acetyl-beta-neuraminate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 38685, electrospray mass spectrometry | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
N-acetylneuraminate mutarotase | - |
Escherichia coli |
sialic acid epimerase | - |
Escherichia coli |
YjhT | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | probably facilitates sialidase-negative bacteria to compete sucessfully for limited amounts of extracellular Neu5Ac, which is likely taken up in he beta-anomer. In addition, the rapid removal of sialic acid from solution might be advantageous to the bacterium to damp down host responses | Escherichia coli |