Cloned (Comment) | Organism |
---|---|
gene HP0859, expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) | Helicobacter pylori |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged enzyme, vapor diffusion technique, mixing of 20 mg/ml protein in 30 mM Tris, pH 7.5, 150 mM NaCl, with precipitant solution containing 0.2 M ammonium sulfate, 0.1 M tri sodium citrate, pH 5.6, 15% w/v PEG 4000, and 5% glycerol, 20°C, X-ray diffraction structure determination and analysis at 2.55 A resolution | Helicobacter pylori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-D-glycero-D-manno-heptose | Helicobacter pylori | - |
ADP-L-glycero-D-manno-heptose | - |
? | |
ADP-D-glycero-D-manno-heptose | Helicobacter pylori G27 | - |
ADP-L-glycero-D-manno-heptose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | B5Z7L9 | gene HP0859 or rfaD | - |
Helicobacter pylori G27 | B5Z7L9 | gene HP0859 or rfaD | - |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gell filtration | Helicobacter pylori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-D-glycero-D-manno-heptose | - |
Helicobacter pylori | ADP-L-glycero-D-manno-heptose | - |
? | |
ADP-D-glycero-D-manno-heptose | - |
Helicobacter pylori G27 | ADP-L-glycero-D-manno-heptose | - |
? |
Subunits | Comment | Organism |
---|---|---|
pentamer | structure modeling of the pentameric enzyme including five protein monomers and 5 NAD+ molecules, overview | Helicobacter pylori |
Synonyms | Comment | Organism |
---|---|---|
ADP-L-glycero-D-manno-heptose-6-epimerase | - |
Helicobacter pylori |
AGME | - |
Helicobacter pylori |
HP0859 | - |
Helicobacter pylori |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | preference of the enzyme for NAD+ compared to NADP+. When NADP is bound, the binding region assumes a different conformation | Helicobacter pylori | |
NAD+ | preferred cofactor compared to NADP+, binds at the enzyme active site, binding site structure, overview | Helicobacter pylori | |
NADP+ | NAD+ is the preferred cofactor | Helicobacter pylori |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Helicobacter pylori | sequence calculation | - |
6.8 |
General Information | Comment | Organism |
---|---|---|
malfunction | a HP0859 knockout mutant shows a severe loss of lipopolysaccharide structure and a significant reduction of adhesion levels in an infection model to human stomach gastric adenocarcinoma AGS cells, if compared with the wild-type strain | Helicobacter pylori |
metabolism | last enzyme in the pathway that produces L-glycero-D-manno-heptose starting from sedoheptulose-7-phosphate | Helicobacter pylori |
additional information | an N-terminal seven-stranded modified Rossmann fold where the NAD+ cofactor is bound and a smaller C-terminal alpha/beta domain are responsible for the binding of the substrate | Helicobacter pylori |