Cloned (Comment) | Organism |
---|---|
mutant enzyme Y140F is expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
Y140F mutant enzyme bound to ADP-beta-D-mannose, hanging drop vapor diffusion method, using 2M (NH4)2SO4, 0.1 M HEPES-Na, pH 7.1, 2% (w7v) PEG 400 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
Y140F | the mutant shows 0.08% epimerase activity compared to the wild type enzyme | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P67910 | - |
- |
Purification (Comment) | Organism |
---|---|
metal affinity column chromatography, hydrophobic affinity column chromatography, and gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-D-glycero-D-mannoheptose | - |
Escherichia coli | ADP-L-glycero-D-mannoheptose | - |
? |
Subunits | Comment | Organism |
---|---|---|
pentamer | x-ray crystallography | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
ADP-L-glycero-D-manno-heptose 6-epimerase | - |
Escherichia coli |
AGME | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | AGME preferentially utilizes NADP+ but can use NAD+ (lower activity) | Escherichia coli | |
NADP+ | AGME preferentially utilizes NADP+ | Escherichia coli |