BRENDA - Enzyme Database
show all sequences of 5.1.3.20

Purification and properties of the Escherichia coli K-12 NAD-dependent nucleotide diphosphosulfate epimerase, ADP-L-glycero-D-mannoheptose 6-epimerase

Ding, L.; Seto, B.L.; Ahmed, S.A.; Coleman, W.G.; J. Biol. Chem. 269, 24384-24390 (1994)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
ADP
-
Escherichia coli
ADPglucose
-
Escherichia coli
ATP
-
Escherichia coli
NADH
-
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
ADP-D-glycero-D-manno-heptose
-
Escherichia coli
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37000
-
6 * 37000, SDS-PAGE
Escherichia coli
230000
250000
gel filtration
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ADP-D-glycero-D-manno-heptose
Escherichia coli
the enzyme is involved in the biosynthesis of ADP-L-glycero-D-mannoheptose, which is a key intermediate of lipopolysaccharide inner core
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Posttranslational Modification
Posttranslational Modification
Commentary
Organism
glycoprotein
-
Escherichia coli
Purification (Commentary)
Commentary
Organism
-
Escherichia coli
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00087
-
-
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ADP-D-glycero-D-manno-heptose
-
2357
Escherichia coli
ADP-L-glycero-D-manno-heptose
-
2357
Escherichia coli
-
ADP-D-glycero-D-manno-heptose
the enzyme is involved in the biosynthesis of ADP-L-glycero-D-mannoheptose, which is a key intermediate of lipopolysaccharide inner core
2357
Escherichia coli
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
hexamer
6 * 37000, SDS-PAGE
Escherichia coli
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
42
-
-
Escherichia coli
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
5
55
5°C: about 45% of maximal activity, 55°C: about 50% of maximal activity
Escherichia coli
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
9.5
-
Escherichia coli
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
contains 1 mol of tightly bound NAD+ per mol of enzyme subunit
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
contains 1 mol of tightly bound NAD+ per mol of enzyme subunit
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ADP
-
Escherichia coli
ADPglucose
-
Escherichia coli
ATP
-
Escherichia coli
NADH
-
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.1
-
ADP-D-glycero-D-manno-heptose
-
Escherichia coli
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37000
-
6 * 37000, SDS-PAGE
Escherichia coli
230000
250000
gel filtration
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ADP-D-glycero-D-manno-heptose
Escherichia coli
the enzyme is involved in the biosynthesis of ADP-L-glycero-D-mannoheptose, which is a key intermediate of lipopolysaccharide inner core
?
-
-
-
Posttranslational Modification (protein specific)
Posttranslational Modification
Commentary
Organism
glycoprotein
-
Escherichia coli
Purification (Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.00087
-
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ADP-D-glycero-D-manno-heptose
-
2357
Escherichia coli
ADP-L-glycero-D-manno-heptose
-
2357
Escherichia coli
-
ADP-D-glycero-D-manno-heptose
the enzyme is involved in the biosynthesis of ADP-L-glycero-D-mannoheptose, which is a key intermediate of lipopolysaccharide inner core
2357
Escherichia coli
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
hexamer
6 * 37000, SDS-PAGE
Escherichia coli
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
42
-
-
Escherichia coli
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
5
55
5°C: about 45% of maximal activity, 55°C: about 50% of maximal activity
Escherichia coli
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
9.5
-
Escherichia coli
Other publictions for EC 5.1.3.20
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
748527
Wang
Construction and characteriza ...
Escherichia coli, Escherichia coli W3110 / K-12
Mar. Drugs
12
1495-1511
2014
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2
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1
1
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726590
Kim
Structure and in silico substr ...
Burkholderia thailandensis, Burkholderia thailandensis E444
Acta Crystallogr. Sect. D
69
658-668
2013
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1
1
2
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1
2
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5
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1
1
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2
2
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2
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2
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2
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1
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727070
Shaik
The crystal structure of ADP-L ...
Helicobacter pylori, Helicobacter pylori G27
Biochim. Biophys. Acta
1814
1641-1647
2011
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1
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5
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1
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3
3
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716848
Kowatz
The crystal structure of the Y ...
Escherichia coli
Protein Sci.
19
1337-1343
2010
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1
1
1
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1
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1
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1
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1
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672239
Morrison
A Two-Base Mechanism for Esche ...
Escherichia coli
Biochemistry
46
3916-3924
2007
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1
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4
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1
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1
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5
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1
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1
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1
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4
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1
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1
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1
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1
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672262
Mayer
Intermediate release by ADP-L- ...
Escherichia coli
Biochemistry
46
6149-6155
2007
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1
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2
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1
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676080
Kim
Role of NtrC in biofilm format ...
Vibrio vulnificus
Mol. Microbiol.
63
559-574
2007
-
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1
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5
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661199
Morrison
Dismutase activity of ADP-L-gl ...
Escherichia coli
Biochemistry
44
5907-5915
2005
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1
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2
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1
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2
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1
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1
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663007
Read
Efficient chemoenzymatic synth ...
Escherichia coli
Org. Lett.
7
2457-2460
2005
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661937
Read
The mechanism of the reaction ...
Escherichia coli
J. Am. Chem. Soc.
126
8878-8879
2004
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652147
Ni
Evidence that NADP+ is the phy ...
Escherichia coli
J. Biol. Chem.
276
27329-27334
2001
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653940
Deacon
The crystal structure of ADP-L ...
Escherichia coli
Structure
8
453-462
2000
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649112
Ding
Crystallization and preliminar ...
Escherichia coli
Acta Crystallogr. Sect. D
55
685-688
1999
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2359
Nichols
Identification of the ADP-L-gl ...
Haemophilus influenzae 2019, Haemophilus influenzae
Infect. Immun.
65
1377-1386
1997
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1
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8
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2358
Drazek
A mutation in the Neisseria go ...
Neisseria gonorrhoeae
J. Bacteriol.
177
2321-2327
1995
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2357
Ding
Purification and properties of ...
Escherichia coli
J. Biol. Chem.
269
24384-24390
1994
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1
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2360
Sirisena
Molecular analysis of the rfaD ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Bacteriol.
176
2379-2385
1994
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2355
Pegues
Cloning, expression, and chara ...
Escherichia coli
J. Bacteriol.
172
4652-4660
1990
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2356
Coleman
The rfaD gene codes for ADP-L- ...
Escherichia coli
J. Biol. Chem.
258
1985-1990
1983
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