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Literature summary for 5.1.3.20 extracted from
- Purification and properties of the Escherichia coli K-12 NAD-dependent nucleotide diphosphosulfate epimerase, ADP-L-glycero-D-mannoheptose 6-epimerase (1994), J. Biol. Chem., 269, 24384-24390.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Escherichia coli |  |
| ADPglucose | - |
Escherichia coli | |
| ATP | - |
Escherichia coli | |
| NADH | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
ADP-D-glycero-D-manno-heptose | - |
Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
6 * 37000, SDS-PAGE | Escherichia coli |
| 230000 | 250000 | gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP-D-glycero-D-manno-heptose | Escherichia coli | the enzyme is involved in the biosynthesis of ADP-L-glycero-D-mannoheptose, which is a key intermediate of lipopolysaccharide inner core | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Escherichia coli |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00087 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP-D-glycero-D-manno-heptose | - |
Escherichia coli | ADP-L-glycero-D-manno-heptose | - |
? | |
| ADP-D-glycero-D-manno-heptose | the enzyme is involved in the biosynthesis of ADP-L-glycero-D-mannoheptose, which is a key intermediate of lipopolysaccharide inner core | Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 37000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
- |
Escherichia coli |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 5 | 55 | 5°C: about 45% of maximal activity, 55°C: about 50% of maximal activity | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9.5 | - |
Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | contains 1 mol of tightly bound NAD+ per mol of enzyme subunit | Escherichia coli | |
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