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Literature summary for 5.1.3.2 extracted from
- Functional characterization and transcriptional analysis of galE gene encoding a UDP-galactose 4-epimerase in Xanthomonas campestris pv. campestris (2013), Microbiol. Res., 169, 441-452.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| galE transcription exhibits a distinct expressionprofile under different culture conditions, mapping of galE transcription initiation | Xanthomonas campestris |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| K151A | site-directed mutagenesis, inactive mutant | Xanthomonas campestris |
| S123A | site-directed mutagenesis, inactive mutant | Xanthomonas campestris |
| Y147A | site-directed mutagenesis, almost inactive mutant | Xanthomonas campestris |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42213 | - |
x * 42213, sequence calculation | Xanthomonas campestris |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose | Xanthomonas campestris | - |
UDP-alpha-D-galactose | - |
r |  |
| UDP-alpha-D-glucose | Xanthomonas campestris Xc17 | - |
UDP-alpha-D-galactose | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Xanthomonas campestris | - |
pv.campestris, gene galE | - |
| Xanthomonas campestris Xc17 | - |
pv.campestris, gene galE | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | galE transcription exhibits a distinct expression profile under different culture conditions | Xanthomonas campestris | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose | - |
Xanthomonas campestris | UDP-alpha-D-galactose | - |
r | |
| UDP-alpha-D-glucose | residues Ser123, Tyr147, Asn177, Asn197, Arg229, Arg290, Asp293,and Tyr297 play a role in UDP-sugar binding | Xanthomonas campestris | UDP-alpha-D-galactose | - |
r | |
| UDP-alpha-D-glucose | - |
Xanthomonas campestris Xc17 | UDP-alpha-D-galactose | - |
r | |
| UDP-alpha-D-glucose | residues Ser123, Tyr147, Asn177, Asn197, Arg229, Arg290, Asp293,and Tyr297 play a role in UDP-sugar binding | Xanthomonas campestris Xc17 | UDP-alpha-D-galactose | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 42213, sequence calculation | Xanthomonas campestris |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GalE | - |
Xanthomonas campestris |
| UDP-galactose 4-epimerase | - |
Xanthomonas campestris |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Xanthomonas campestris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Xanthomonas campestris |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | the amino acids that interact with NADH are Asp31, Asn35, Ser36, Lys83, Asn98, Tyr147, and Lys151 | Xanthomonas campestris | |
| NADH | the amino acids that interact with NADH are Asp31, Asn35, Ser36, Lys83, Asn98, Tyr147, and Lys151 | Xanthomonas campestris | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Xanthomonas campestris | gene galE is subject to catabolite repression | down |
| Xanthomonas campestris | gene galE is positively regulatedby Clp and RpfF | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the GalE enzyme is a member of the extended short-chain dehydrogenase/reductase superfamily of proteins. It has the two signature sequences of the extended SDR superfamily, a GxxGxxG motif, which is located near the cofactor binding pocket, and a YxxxK motif, in which the conserved tyrosine plays a key role in catalysis, are strictly conserved in Xanthomonas GalE as well as several crystallized GalE proteins from other bacteria | Xanthomonas campestris |
| malfunction | a Xcc galE mutant has reduced biofilm formation ability | Xanthomonas campestris |
| metabolism | the galE gene product is not the only enzyme responsible for UDP-glucose production in the organism | Xanthomonas campestris |
| additional information | putative GalE catalytic residues are Ser124, Tyr147, and Lys151 | Xanthomonas campestris |
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