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Literature summary for 5.1.3.2 extracted from

  • Agarwal, S.; Gopal, K.; Upadhyaya, T.; Dixit, A.
    Biochemical and functional characterization of UDP-galactose 4-epimerase from Aeromonas hydrophila (2007), Biochim. Biophys. Acta, 1774, 828-837.
    View publication on PubMed

Application

Application Comment Organism
medicine drug design Aeromonas hydrophila

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Aeromonas hydrophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
UDP-galactose
-
Aeromonas hydrophila

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
MALDI-TOF MS Aeromonas hydrophila
38270
-
MALDI-TOF MS Aeromonas hydrophila

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Aeromonas hydrophila Leloir pathway of galactose metabolism ?
-
?
UDP-galactose Aeromonas hydrophila
-
UDP-glucose
-
r

Organism

Organism UniProt Comment Textmining
Aeromonas hydrophila
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose anion exchange chromatography Aeromonas hydrophila
Ni2+-NTA affinity chromatography Aeromonas hydrophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Leloir pathway of galactose metabolism Aeromonas hydrophila ?
-
?
UDP-galactose
-
Aeromonas hydrophila UDP-glucose
-
r

Subunits

Subunits Comment Organism
monomer
-
Aeromonas hydrophila

Synonyms

Synonyms Comment Organism
6xHis-rGalE
-
Aeromonas hydrophila
GalE
-
Aeromonas hydrophila
rGalE
-
Aeromonas hydrophila
UDP-galactose 4-epimerase
-
Aeromonas hydrophila

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Aeromonas hydrophila

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 45
-
Aeromonas hydrophila

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
12.9
-
UDP-galactose
-
Aeromonas hydrophila

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Aeromonas hydrophila
8 9
-
Aeromonas hydrophila

pH Range

pH Minimum pH Maximum Comment Organism
3 10
-
Aeromonas hydrophila