Literature summary for 5.1.3.2 extracted from

Thoden, J.B.; Wohlers, T.M.; Fridovich-Keil, J.L.; Holden, H.M.
Crystallographic evidence for Tyr 157 functioning as the active site base in human UDP-galactose 4-epimerase (2000), Biochemistry, 39, 5691-5701.

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Pichia pastoris	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
suitable crystallization conditions are both at room temperature and at 4°C using sparse matrix screen and the hanging drop method of vapor diffusion. The best crystals are observed growing from polyethylene glycol 8000 and potassium chloride and in the presence of 2 mM NAD+. Typical large crystals are ontained from 18-20% polyethylene glycol 8000, 100 mM Hepes, pH 7.5, and 250 mM KCl at room temperature, crystallization of a epimerase/NADH/UDP-glucose ternary complex	Homo sapiens

Crystallization (Comment)

Organism

suitable crystallization conditions are both at room temperature and at 4°C using sparse matrix screen and the hanging drop method of vapor diffusion. The best crystals are observed growing from polyethylene glycol 8000 and potassium chloride and in the presence of 2 mM NAD+. Typical large crystals are ontained from 18-20% polyethylene glycol 8000, 100 mM Hepes, pH 7.5, and 250 mM KCl at room temperature, crystallization of a epimerase/NADH/UDP-glucose ternary complex

Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	catalyzes the interconversion of UDP-glucose and UDP-galactose during normal galactose metabolism	Homo sapiens	?	-	?

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	the amino acid side chains responsible for anchoring the NAD+ to the protein include Asp33, Asn37, Asp66, Tyr157 and Lys161	Homo sapiens

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Release 2023.1 (January 2023)