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Literature summary for 5.1.3.2 extracted from
- Reversible folding of UDP-galactose-4-epimerase from yeast Kluyveromyces fragilis (1993), Biochemistry, 32, 9726-9734.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
UDPgalactose | native and renatured enzyme | Kluyveromyces marxianus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 158000 | - |
gel filtration | Kluyveromyces marxianus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Kluyveromyces marxianus | - |
- |
- |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| denaturation in presence of 8 M urea. Dilution of the denaturant by sodium phosphate buffer, 20 mM, pH 7.0, containing 1 mM NAD+ recovers the activity to the extent of 80-100% | Kluyveromyces marxianus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-galactose | - |
Kluyveromyces marxianus | UDP-glucose | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
pH 7.0, 4 h stable, native enzyme | Kluyveromyces marxianus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | contains one molecule of NAD+ per dimer | Kluyveromyces marxianus | |
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