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Literature summary for 5.1.3.18 extracted from
- Characterization of a GDP-d-mannose 3'',5''-epimerase from rice (2006), Phytochemistry, 67, 338-346.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | accelerates activity | Oryza sativa |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression as a fusion protein in Escherichia coli | Oryza sativa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| GDP | - |
Oryza sativa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00712 | - |
GDP-D-mannose | without NAD+ | Oryza sativa | |
| 0.012 | - |
GDP-D-mannose | in presence of 0.02 mM NAD+ | Oryza sativa | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-D-mannose | Oryza sativa | key enzyme in biosynthesis of vitamin C in plants | GDP-L-galactose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryza sativa | A3C4S4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant | Oryza sativa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDP-D-mannose | key enzyme in biosynthesis of vitamin C in plants | Oryza sativa | GDP-L-galactose | - |
? | |
| GDP-D-mannose | - |
Oryza sativa | GDP-L-galactose + GDP-L-gulose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GDP-D-mannose 3'',5''-epimerase | - |
Oryza sativa |
| OsGME | - |
Oryza sativa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
without NAD+ | Oryza sativa |
| 25 | - |
in presence of NAD+ | Oryza sativa |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
GDP-D-mannose | without NAD+ | Oryza sativa | |
| 0.127 | - |
GDP-D-mannose | in presence of 0.02 mM NAD+ | Oryza sativa | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | in absence of NAD+ | Oryza sativa |
| 8 | 8.5 | in presence of NAD+ | Oryza sativa |
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