Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.1.3.17 extracted from

  • Zhang, J.; Suflita, M.; Li, G.; Zhong, W.; Li, L.; Dordick, J.S.; Linhardt, R.J.; Zhang, F.
    High cell density cultivation of recombinant Escherichia coli strains expressing 2-O-sulfotransferase and C5-epimerase for the production of bioengineered heparin (2015), Appl. Biochem. Biotechnol., 175, 2986-2995 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis the enzyme, coexpressed with 2-O-sulfotransferase in bacteria, can be used for production of bioengineered heparin as a potential substitute for the animal-sourced anticoagulant drug Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
recombinant MBP-tagged enzyme in Escherichia coli strain Rosetta-gami B (DE3), coexpression with 2-O-sulfotransferase Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information high cell density fed-batch cultivation of recombinant Escherichia coli strains expressing 2-O-sulfotransferase and C5-epimerase at high level for the production of bioengineered heparin, method, overview. The first enzymatic step in this process uses heparan sulfate biosynthetic enzymes, 2-O-sulfotransferase (2-OST) and C5-epimerase (C5-epi), expressed as MBP-tagged proteins in Escherichia coli, to convert N-sulfo heparosan into an intermediate polysaccharide rich in -GlcNS(1->4)IdoA2S- sequences (where S is sulfo and IdoA is alpha-L-iduronic acid). This critical step in bioengineered heparin preparation relies on the use of recombinant arylsulfotransferase IV (AST-IV) to regenerate 3'-phospho adenosine-5'-phosphosulfate (PAPS) using p-nitrophenylsulfate as a sacrificial sulfur donor, one-pot reaction Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens O94923
-
-

Synonyms

Synonyms Comment Organism
C5-epi
-
Homo sapiens
Glce
-
Homo sapiens
HS C5-epimerase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Homo sapiens