Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KCl | stimulates, maximal effect at 0.1 M, inhibition at higher concentrations | Bos taurus | |
NaCl | stimulates, maximal effect at 0.05 M, inhibition at higher concentrations | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.063 | - |
uronic acid residues | - |
Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no specific metal ion requirement | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
x * 52000, liver, SDS-PAGE | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Bos taurus | enzyme is involved in the biosynthesis of heparan sulfate in liver | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Bos taurus |
Storage Stability | Organism |
---|---|
4°C, 10% glycerol, retains full activity for 1.5 years | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Heparosan-N-sulfate D-glucuronate | heparosan N-sulfate prepared by metabolic labeling of a capsular polysaccharide from E. coli K5 and subsequent chemical partial N-deacetylation and N-sulfation. Approximately 30% of the D-glucuronyl residues located between two N-sulfated glucosamine units are converted to L-iduronyl units | Bos taurus | Heparosan-N-sulfate L-iduronate | - |
? | |
additional information | enzyme is involved in the biosynthesis of heparan sulfate in liver | Bos taurus | ? | - |
? | |
uronic acid residues | - |
Bos taurus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52000, liver, SDS-PAGE | Bos taurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
620 | - |
uronic acid residues | - |
Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 7.5 | - |
Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.1 | 7.9 | pH 6.1: about 60% of maximal activity, pH 7.9: about 30% of maximal activity | Bos taurus |