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show all sequences of 5.1.3.14

Characterizing non-hydrolyzing Neisseria meningitidis serogroup A UDP-N-acetylglucosamine (UDP-GlcNAc) 2-epimerase using UDP-N-acetylmannosamine (UDP-ManNAc) and derivatives

Zhang, L.; Muthana, M.M.; Yu, H.; McArthur, J.B.; Qu, J.; Chen, X.; Carbohydr. Res. 419, 18-28 (2016)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
no activation by EDTA, and 1 mM DTT
Neisseria meningitidis serogroup A / serotype 4A
UDP-GlcNAc
allosteric activator, absolutely required for their UDP-ManNAc 2-epimerization catalytic activity, activates recombinant His6-tagged enzyme NmSacA
Neisseria meningitidis serogroup A / serotype 4A
Cloned(Commentary)
Commentary
Organism
gene sacA, encoded in the serogroup A cps operon, sequence comparisons, recombinant overexpression of C-terminally His6-tagged recombinant NmSacA in Escherichia coli strain BL21(DE3)
Neisseria meningitidis serogroup A / serotype 4A
Inhibitors
Inhibitors
Commentary
Organism
Structure
2-acetamidoglucal
-
Neisseria meningitidis serogroup A / serotype 4A
Ca2+
-
Neisseria meningitidis serogroup A / serotype 4A
Cu2+
-
Neisseria meningitidis serogroup A / serotype 4A
Mg2+
-
Neisseria meningitidis serogroup A / serotype 4A
Mn2+
leads to enzyme precipitation at 10 mM
Neisseria meningitidis serogroup A / serotype 4A
additional information
both 2-acetamidoglucal and UDP show competitive inhibitory effects for the UDP-ManNAc 2-epimerization of NmSacA-His6. No inhibition by EDTA, and 1 mM DTT
Neisseria meningitidis serogroup A / serotype 4A
Ni2+
-
Neisseria meningitidis serogroup A / serotype 4A
UDP
-
Neisseria meningitidis serogroup A / serotype 4A
Zn2+
leads to enzyme precipitation at 10 mM
Neisseria meningitidis serogroup A / serotype 4A
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6
-
UDP-N-acetyl-alpha-D-glucosamine
pH 8.5, 37°C, recombinant enzyme
Neisseria meningitidis serogroup A / serotype 4A
5.2
-
UDP-N-acetyl-alpha-D-mannosamine
pH 8.5, 37°C, recombinant enzyme
Neisseria meningitidis serogroup A / serotype 4A
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
the 2-epimerase activity of NmSacA-His6 is not dependent on the addition of an external divalent metal ion, Li+ and EDTA have no effect on enzyme activity
Neisseria meningitidis serogroup A / serotype 4A
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-N-acetyl-alpha-D-glucosamine
Neisseria meningitidis serogroup A / serotype 4A
-
UDP-N-acetyl-alpha-D-mannosamine
-
-
r
UDP-N-acetyl-alpha-D-glucosamine
Neisseria meningitidis serogroup A / serotype 4A Z2491
-
UDP-N-acetyl-alpha-D-mannosamine
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Neisseria meningitidis serogroup A / serotype 4A
A0A0U1RGY0
-
-
Neisseria meningitidis serogroup A / serotype 4A Z2491
A0A0U1RGY0
-
-
Purification (Commentary)
Commentary
Organism
recombinant C-terminally His6-tagged NmSacA from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Neisseria meningitidis serogroup A / serotype 4A
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dihydropyrimidine-2,4(1H,3H)-dione-alpha-D-ribofuranosyl-5'-diphospho-2-acetamido-2-deoxy-alpha-D-mannopyranoside
-
747462
Neisseria meningitidis serogroup A / serotype 4A
?
-
-
-
?
dihydropyrimidine-2,4(1H,3H)-dione-alpha-D-ribofuranosyl-5'-diphospho-2-acetamido-2-deoxy-alpha-D-mannopyranoside
-
747462
Neisseria meningitidis serogroup A / serotype 4A Z2491
?
-
-
-
?
additional information
substrate specificity, overview. Recombinant His6-tagged NmSacA-His6 can tolerate several chemoenzymatically synthesized UDP-ManNAc derivatives as substrates in the absence of UDP-GlcNAc although its activity is much lower than with non-modified UDP-ManNAc. Homology modeling and molecular docking. The formation of UDP-GlcNAc or UDP-ManNAc is not observed by incubating NmSacA-His6 with 2-acetamidoglucal and UDP in contrast to the serogroup B enzyme. No enzyme activity with UDP-mannose, UDP-ManF, UDP-ManN3, UDP-ManNH2, uridine 5'-diphospho-2-butyramido-2-deoxy-alpha-D-mannopyranoside, uridine 5'-diphospho-2-azidoacetamido-2-deoxy-alpha-D-mannopyranoside, uridine 5'-diphospho-2-phenylacetamido-2-deoxy-alpha-D-mannopyranoside, dihydropyrimidine-2, 4(1H,3H)-dione-alpha-D-ribofuranosyl-5'-diphospho-2-azidoxyacetamido-2-deoxy-alpha-D-mannopyranoside, and uridine 5'-diphospho-2-azidoacetamido-2-deoxy-alpha-D-glucopyranoside, docking study
747462
Neisseria meningitidis serogroup A / serotype 4A
?
-
-
-
-
additional information
substrate specificity, overview. Recombinant His6-tagged NmSacA-His6 can tolerate several chemoenzymatically synthesized UDP-ManNAc derivatives as substrates in the absence of UDP-GlcNAc although its activity is much lower than with non-modified UDP-ManNAc. Homology modeling and molecular docking. The formation of UDP-GlcNAc or UDP-ManNAc is not observed by incubating NmSacA-His6 with 2-acetamidoglucal and UDP in contrast to the serogroup B enzyme. No enzyme activity with UDP-mannose, UDP-ManF, UDP-ManN3, UDP-ManNH2, uridine 5'-diphospho-2-butyramido-2-deoxy-alpha-D-mannopyranoside, uridine 5'-diphospho-2-azidoacetamido-2-deoxy-alpha-D-mannopyranoside, uridine 5'-diphospho-2-phenylacetamido-2-deoxy-alpha-D-mannopyranoside, dihydropyrimidine-2, 4(1H,3H)-dione-alpha-D-ribofuranosyl-5'-diphospho-2-azidoxyacetamido-2-deoxy-alpha-D-mannopyranoside, and uridine 5'-diphospho-2-azidoacetamido-2-deoxy-alpha-D-glucopyranoside, docking study
747462
Neisseria meningitidis serogroup A / serotype 4A Z2491
?
-
-
-
-
UDP-N-acetyl-alpha-D-glucosamine
-
747462
Neisseria meningitidis serogroup A / serotype 4A
UDP-N-acetyl-alpha-D-mannosamine
-
-
-
r
UDP-N-acetyl-alpha-D-glucosamine
-
747462
Neisseria meningitidis serogroup A / serotype 4A Z2491
UDP-N-acetyl-alpha-D-mannosamine
-
-
-
r
UDP-N-acetyl-alpha-D-mannosamine
-
747462
Neisseria meningitidis serogroup A / serotype 4A
UDP-N-acetyl-alpha-D-glucosamine
-
-
-
r
UDP-N-acetyl-alpha-D-mannosamine
-
747462
Neisseria meningitidis serogroup A / serotype 4A Z2491
UDP-N-acetyl-alpha-D-glucosamine
-
-
-
r
uridine 5'-diphospho-2-hydroxyacetamido-2-deoxy-alpha-D-mannopyranoside
-
747462
Neisseria meningitidis serogroup A / serotype 4A
?
-
-
-
?
uridine 5'-diphospho-2-hydroxyacetamido-2-deoxy-alpha-D-mannopyranoside
-
747462
Neisseria meningitidis serogroup A / serotype 4A Z2491
?
-
-
-
?
uridine 5'-diphospho-2-propionamido-2-deoxy-alpha-D-mannopyranoside
-
747462
Neisseria meningitidis serogroup A / serotype 4A
?
-
-
-
?
uridine 5'-diphospho-N-trifluoroaceto-2-deoxy-alpha-D-mannopyranoside
-
747462
Neisseria meningitidis serogroup A / serotype 4A
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 43000, recombinant His6-tagged NmSacA, SDS-PAGE
Neisseria meningitidis serogroup A / serotype 4A
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Neisseria meningitidis serogroup A / serotype 4A
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
31
-
UDP-N-acetyl-alpha-D-glucosamine
pH 8.5, 37°C, recombinant enzyme
Neisseria meningitidis serogroup A / serotype 4A
52
-
UDP-N-acetyl-alpha-D-mannosamine
pH 8.5, 37°C, recombinant enzyme
Neisseria meningitidis serogroup A / serotype 4A
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
9
-
Neisseria meningitidis serogroup A / serotype 4A
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
11
activity range with high activity
Neisseria meningitidis serogroup A / serotype 4A
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.8
-
UDP
pH 8.5, 37°C, recombinant enzyme
Neisseria meningitidis serogroup A / serotype 4A
14
-
2-acetamidoglucal
pH 8.5, 37°C, recombinant enzyme
Neisseria meningitidis serogroup A / serotype 4A
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
no activation by EDTA, and 1 mM DTT
Neisseria meningitidis serogroup A / serotype 4A
UDP-GlcNAc
allosteric activator, absolutely required for their UDP-ManNAc 2-epimerization catalytic activity, activates recombinant His6-tagged enzyme NmSacA
Neisseria meningitidis serogroup A / serotype 4A
Cloned(Commentary) (protein specific)
Commentary
Organism
gene sacA, encoded in the serogroup A cps operon, sequence comparisons, recombinant overexpression of C-terminally His6-tagged recombinant NmSacA in Escherichia coli strain BL21(DE3)
Neisseria meningitidis serogroup A / serotype 4A
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-acetamidoglucal
-
Neisseria meningitidis serogroup A / serotype 4A
Ca2+
-
Neisseria meningitidis serogroup A / serotype 4A
Cu2+
-
Neisseria meningitidis serogroup A / serotype 4A
Mg2+
-
Neisseria meningitidis serogroup A / serotype 4A
Mn2+
leads to enzyme precipitation at 10 mM
Neisseria meningitidis serogroup A / serotype 4A
additional information
both 2-acetamidoglucal and UDP show competitive inhibitory effects for the UDP-ManNAc 2-epimerization of NmSacA-His6. No inhibition by EDTA, and 1 mM DTT
Neisseria meningitidis serogroup A / serotype 4A
Ni2+
-
Neisseria meningitidis serogroup A / serotype 4A
UDP
-
Neisseria meningitidis serogroup A / serotype 4A
Zn2+
leads to enzyme precipitation at 10 mM
Neisseria meningitidis serogroup A / serotype 4A
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.8
-
UDP
pH 8.5, 37°C, recombinant enzyme
Neisseria meningitidis serogroup A / serotype 4A
14
-
2-acetamidoglucal
pH 8.5, 37°C, recombinant enzyme
Neisseria meningitidis serogroup A / serotype 4A
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.6
-
UDP-N-acetyl-alpha-D-glucosamine
pH 8.5, 37°C, recombinant enzyme
Neisseria meningitidis serogroup A / serotype 4A
5.2
-
UDP-N-acetyl-alpha-D-mannosamine
pH 8.5, 37°C, recombinant enzyme
Neisseria meningitidis serogroup A / serotype 4A
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
the 2-epimerase activity of NmSacA-His6 is not dependent on the addition of an external divalent metal ion, Li+ and EDTA have no effect on enzyme activity
Neisseria meningitidis serogroup A / serotype 4A
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
UDP-N-acetyl-alpha-D-glucosamine
Neisseria meningitidis serogroup A / serotype 4A
-
UDP-N-acetyl-alpha-D-mannosamine
-
-
r
UDP-N-acetyl-alpha-D-glucosamine
Neisseria meningitidis serogroup A / serotype 4A Z2491
-
UDP-N-acetyl-alpha-D-mannosamine
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant C-terminally His6-tagged NmSacA from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Neisseria meningitidis serogroup A / serotype 4A
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dihydropyrimidine-2,4(1H,3H)-dione-alpha-D-ribofuranosyl-5'-diphospho-2-acetamido-2-deoxy-alpha-D-mannopyranoside
-
747462
Neisseria meningitidis serogroup A / serotype 4A
?
-
-
-
?
dihydropyrimidine-2,4(1H,3H)-dione-alpha-D-ribofuranosyl-5'-diphospho-2-acetamido-2-deoxy-alpha-D-mannopyranoside
-
747462
Neisseria meningitidis serogroup A / serotype 4A Z2491
?
-
-
-
?
additional information
substrate specificity, overview. Recombinant His6-tagged NmSacA-His6 can tolerate several chemoenzymatically synthesized UDP-ManNAc derivatives as substrates in the absence of UDP-GlcNAc although its activity is much lower than with non-modified UDP-ManNAc. Homology modeling and molecular docking. The formation of UDP-GlcNAc or UDP-ManNAc is not observed by incubating NmSacA-His6 with 2-acetamidoglucal and UDP in contrast to the serogroup B enzyme. No enzyme activity with UDP-mannose, UDP-ManF, UDP-ManN3, UDP-ManNH2, uridine 5'-diphospho-2-butyramido-2-deoxy-alpha-D-mannopyranoside, uridine 5'-diphospho-2-azidoacetamido-2-deoxy-alpha-D-mannopyranoside, uridine 5'-diphospho-2-phenylacetamido-2-deoxy-alpha-D-mannopyranoside, dihydropyrimidine-2, 4(1H,3H)-dione-alpha-D-ribofuranosyl-5'-diphospho-2-azidoxyacetamido-2-deoxy-alpha-D-mannopyranoside, and uridine 5'-diphospho-2-azidoacetamido-2-deoxy-alpha-D-glucopyranoside, docking study
747462
Neisseria meningitidis serogroup A / serotype 4A
?
-
-
-
-
additional information
substrate specificity, overview. Recombinant His6-tagged NmSacA-His6 can tolerate several chemoenzymatically synthesized UDP-ManNAc derivatives as substrates in the absence of UDP-GlcNAc although its activity is much lower than with non-modified UDP-ManNAc. Homology modeling and molecular docking. The formation of UDP-GlcNAc or UDP-ManNAc is not observed by incubating NmSacA-His6 with 2-acetamidoglucal and UDP in contrast to the serogroup B enzyme. No enzyme activity with UDP-mannose, UDP-ManF, UDP-ManN3, UDP-ManNH2, uridine 5'-diphospho-2-butyramido-2-deoxy-alpha-D-mannopyranoside, uridine 5'-diphospho-2-azidoacetamido-2-deoxy-alpha-D-mannopyranoside, uridine 5'-diphospho-2-phenylacetamido-2-deoxy-alpha-D-mannopyranoside, dihydropyrimidine-2, 4(1H,3H)-dione-alpha-D-ribofuranosyl-5'-diphospho-2-azidoxyacetamido-2-deoxy-alpha-D-mannopyranoside, and uridine 5'-diphospho-2-azidoacetamido-2-deoxy-alpha-D-glucopyranoside, docking study
747462
Neisseria meningitidis serogroup A / serotype 4A Z2491
?
-
-
-
-
UDP-N-acetyl-alpha-D-glucosamine
-
747462
Neisseria meningitidis serogroup A / serotype 4A
UDP-N-acetyl-alpha-D-mannosamine
-
-
-
r
UDP-N-acetyl-alpha-D-glucosamine
-
747462
Neisseria meningitidis serogroup A / serotype 4A Z2491
UDP-N-acetyl-alpha-D-mannosamine
-
-
-
r
UDP-N-acetyl-alpha-D-mannosamine
-
747462
Neisseria meningitidis serogroup A / serotype 4A
UDP-N-acetyl-alpha-D-glucosamine
-
-
-
r
UDP-N-acetyl-alpha-D-mannosamine
-
747462
Neisseria meningitidis serogroup A / serotype 4A Z2491
UDP-N-acetyl-alpha-D-glucosamine
-
-
-
r
uridine 5'-diphospho-2-hydroxyacetamido-2-deoxy-alpha-D-mannopyranoside
-
747462
Neisseria meningitidis serogroup A / serotype 4A
?
-
-
-
?
uridine 5'-diphospho-2-hydroxyacetamido-2-deoxy-alpha-D-mannopyranoside
-
747462
Neisseria meningitidis serogroup A / serotype 4A Z2491
?
-
-
-
?
uridine 5'-diphospho-2-propionamido-2-deoxy-alpha-D-mannopyranoside
-
747462
Neisseria meningitidis serogroup A / serotype 4A
?
-
-
-
?
uridine 5'-diphospho-N-trifluoroaceto-2-deoxy-alpha-D-mannopyranoside
-
747462
Neisseria meningitidis serogroup A / serotype 4A
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 43000, recombinant His6-tagged NmSacA, SDS-PAGE
Neisseria meningitidis serogroup A / serotype 4A
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Neisseria meningitidis serogroup A / serotype 4A
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
31
-
UDP-N-acetyl-alpha-D-glucosamine
pH 8.5, 37°C, recombinant enzyme
Neisseria meningitidis serogroup A / serotype 4A
52
-
UDP-N-acetyl-alpha-D-mannosamine
pH 8.5, 37°C, recombinant enzyme
Neisseria meningitidis serogroup A / serotype 4A
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
9
-
Neisseria meningitidis serogroup A / serotype 4A
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
11
activity range with high activity
Neisseria meningitidis serogroup A / serotype 4A
General Information
General Information
Commentary
Organism
additional information
of the six major disease-causing Neisseria meningitidis serogroups, only serogroups A and X produce capsular polysaccharides (CPSs) that do not have N-acetylneuraminic acid (Neu5Ac, sialic acid is a more general term) residues. Unlike the CPSs of serogroups B and C which are homopolymers of Neu5Ac with alpha2-8- and alpha2-9-linkages respectively, or serogroups W-135 and Y CPSs which are heteropolymers of [-6Gal/Glcalpha1-4Neu5Acalpha2-]n with alternating Neu5Ac and Gal/Glc as disaccharide repeating units, the CPS of serogroup A is a homopolymer of [-6ManNAcalpha1-phosphate-]n. Correspondingly, the genetic organization for serogroup A capsule is different from those for serogroups B, C, W-135, and Y
Neisseria meningitidis serogroup A / serotype 4A
physiological function
SacA is proposed to be involved in the first step in serogroup A capsular polysaccharides (CPS) biosynthetic pathway and is critical for serogroup A CPS biosynthesis. It catalyzes the interconversion between UDP-GlcNAc and UDP-ManNAc. This epimerization process is independent of nicotinamide adenine dinucleotide oxidized form (NAD+) and is critical for synthesizing bacterial ManNAc-containing CPSs
Neisseria meningitidis serogroup A / serotype 4A
General Information (protein specific)
General Information
Commentary
Organism
additional information
of the six major disease-causing Neisseria meningitidis serogroups, only serogroups A and X produce capsular polysaccharides (CPSs) that do not have N-acetylneuraminic acid (Neu5Ac, sialic acid is a more general term) residues. Unlike the CPSs of serogroups B and C which are homopolymers of Neu5Ac with alpha2-8- and alpha2-9-linkages respectively, or serogroups W-135 and Y CPSs which are heteropolymers of [-6Gal/Glcalpha1-4Neu5Acalpha2-]n with alternating Neu5Ac and Gal/Glc as disaccharide repeating units, the CPS of serogroup A is a homopolymer of [-6ManNAcalpha1-phosphate-]n. Correspondingly, the genetic organization for serogroup A capsule is different from those for serogroups B, C, W-135, and Y
Neisseria meningitidis serogroup A / serotype 4A
physiological function
SacA is proposed to be involved in the first step in serogroup A capsular polysaccharides (CPS) biosynthetic pathway and is critical for serogroup A CPS biosynthesis. It catalyzes the interconversion between UDP-GlcNAc and UDP-ManNAc. This epimerization process is independent of nicotinamide adenine dinucleotide oxidized form (NAD+) and is critical for synthesizing bacterial ManNAc-containing CPSs
Neisseria meningitidis serogroup A / serotype 4A
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
8.6
-
UDP-N-acetyl-alpha-D-mannosamine
pH 8.5, 37°C, recombinant enzyme
Neisseria meningitidis serogroup A / serotype 4A
10
-
UDP-N-acetyl-alpha-D-glucosamine
pH 8.5, 37°C, recombinant enzyme
Neisseria meningitidis serogroup A / serotype 4A
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
8.6
-
UDP-N-acetyl-alpha-D-mannosamine
pH 8.5, 37°C, recombinant enzyme
Neisseria meningitidis serogroup A / serotype 4A
10
-
UDP-N-acetyl-alpha-D-glucosamine
pH 8.5, 37°C, recombinant enzyme
Neisseria meningitidis serogroup A / serotype 4A
Other publictions for EC 5.1.3.14
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Novel GNE mutations in heredit ...
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Lessons from GNE-deficient emb ...
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Molecular modeling of the bifu ...
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UDP-N-acetylglucosamine 2-epim ...
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Enhanced sialylation of EPO by ...
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Sparks
Use of a cell-free system to d ...
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Glycobiology
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Murkin
Identification and mechanism o ...
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Biochemistry
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Active site mutants of the 'no ...
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Biochim. Biophys. Acta
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Giordanengo
Epigenetic reprogramming of UD ...
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FASEB J.
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Biosynthesis of uridine diphos ...
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Biochem. Biophys. Res. Commun.
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1506-1512
1975
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2261
Kikuchi
Purification and properties of ...
Rattus norvegicus
Biochim. Biophys. Acta
327
193-206
1973
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2259
Sommar
Uridine diphosphate N-acetyl-D ...
Rattus norvegicus
Biochim. Biophys. Acta
268
581-589
1972
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2260
Sommar
Uridine diphosphate N-acetyl-D ...
Rattus norvegicus
Biochim. Biophys. Acta
268
590-595
1972
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2258
Salo
Studies on the mechanism of ac ...
Rattus norvegicus
Biochemistry
9
882-885
1970
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2257
Spivak
-
UDP-N-acetyl-D-glucosamine 2'- ...
Rattus norvegicus
Methods Enzymol.
9
612-615
1966
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