Activating Compound | Comment | Organism | Structure |
---|---|---|---|
UDP-N-acetyl-D-glucosamine | allosteric activation | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
wild-type and mutant enzymes expressed in Escherichia coli | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D95N | about 18000 fold decrease in turnover number for UDP-N-acetyl-D-glucosamine, not possible to obtain accurate kinetic constants | Escherichia coli |
E117Q | about 18000 fold decrease in turnover number for UDP-N-acetyl-D-glucosamine, not possible to obtain accurate kinetic constants | Escherichia coli |
E131Q | about 18000 fold decrease in turnover number for UDP-N-acetyl-D-glucosamine, not possible to obtain accurate kinetic constants | Escherichia coli |
H213N | 30fold increase in Km-value and 50fold decrease in turnover-number for UDP-N-acetyl-D-glucosamine. Unlike the wild-type enzyme no inhibition is detected at UDP-concentrations up to 10 mM | Escherichia coli |
K15A | more than 100fold increase in KM-value for UDP-N-acetyl-D-glucosamine | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
UDP-N-acetyl-D-glucosamine | 37°C, pH 8.8, wild-type enzyme | Escherichia coli | |
17.2 | - |
UDP-N-acetyl-D-glucosamine | 37°C, pH 8.8, muatnt enzyme H213N | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes, His-tagged proteins | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine | - |
Escherichia coli | UDP-N-acetyl-D-mannosamine | - |
r |
Synonyms | Comment | Organism |
---|---|---|
non-hydrolyzing UDP-N-acetylglucosamine 2-epimerase | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
UDP-N-acetyl-D-glucosamine | 37°C, pH 8.8, muatnt enzyme H213N | Escherichia coli | |
7.1 | - |
UDP-N-acetyl-D-glucosamine | 37°C, pH 8.8, wild-type enzyme | Escherichia coli |