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Literature summary for 5.1.3.14 extracted from

  • Murkin, A.S.; Chou, W.K.; Wakarchuk, W.W.; Tanner, M.E.
    Identification and mechanism of a bacterial hydrolyzing UDP-N-acetylglucosamine 2-epimerase (2004), Biochemistry, 43, 14290-14298.
    View publication on PubMed
Search for more literature for 5.1.3.14

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli BL21(DE3), wild-type enzyme and mutant enzymes D100N, E122Q and D131N Neisseria meningitidis

Protein Variants

Protein Variants Comment Organism
D100N no conversion of UDP-N-acetyl-D-glucosamine to UDP + N-acetyl-D-mannosamine Neisseria meningitidis
D131N no conversion of UDP-N-acetyl-D-glucosamine to UDP + N-acetyl-D-mannosamine, acetamidoglucal is released from the active site during catalysis Neisseria meningitidis
E122Q no conversion of UDP-N-acetyl-D-glucosamine to UDP + N-acetyl-D-mannosamine, acetamidoglucal is released from the active site during catalysis Neisseria meningitidis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-D-glucosamine Neisseria meningitidis first step of sialic acid biosynthesis UDP-N-acetyl-D-mannosamine
-
 ?
UDP-N-acetyl-D-glucosamine Neisseria meningitidis MC58 group B first step of sialic acid biosynthesis UDP-N-acetyl-D-mannosamine
-
 ?

Organism

Organism UniProt Comment Textmining
Neisseria meningitidis
-
 MC58 group B
-

Purification (Commentary)

Purification (Comment) Organism
-
 Neisseria meningitidis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-D-glucosamine first step of sialic acid biosynthesis Neisseria meningitidis UDP-N-acetyl-D-mannosamine
-
 ?
UDP-N-acetyl-D-glucosamine mechanism involves an anti elimination of UDP to form 2-acetamidoglucal as an intermediate, followed by syn-addition of water Neisseria meningitidis UDP-N-acetyl-D-mannosamine
-
 ?
UDP-N-acetyl-D-glucosamine first step of sialic acid biosynthesis Neisseria meningitidis MC58 group B UDP-N-acetyl-D-mannosamine
-
 ?
UDP-N-acetyl-D-glucosamine mechanism involves an anti elimination of UDP to form 2-acetamidoglucal as an intermediate, followed by syn-addition of water Neisseria meningitidis MC58 group B UDP-N-acetyl-D-mannosamine
-
 ?