Cloned (Comment) | Organism |
---|---|
high level overexpression of the active enzyme is established by using the baculovirus/Sf9 system | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
D413K | enzyme with mutation in the putative kinase active site shows drastic loss in their kinase activity but retains their epimerase activity | Rattus norvegicus |
D413N | enzyme with mutation in the putative kinase active site shows drastic loss in their kinase activity but retains their epimerase activity | Rattus norvegicus |
H110A | mutant enzyme shows a drastic loss of epimerase activity, oligomerization is significantly different from that of the wild-type enzyme,loss of epimerase activity can largely by attributed to incorrect protein folding | Rattus norvegicus |
H132A | mutant enzyme shows a drastic loss of epimerase activity, oligomerization is significantly different from that of the wild-type enzyme, loss of epimerase activity can largely by attributed to incorrect protein folding | Rattus norvegicus |
H155A | mutant enzyme forms mainly trimeric enzyme with small amounts of hexamer | Rattus norvegicus |
H155A | mutant enzyme shows a drastic loss of epimerase activity, loss of epimerase activity can largely by attributed to incorrect protein folding | Rattus norvegicus |
H157A | mutant enzyme forms mainly trimeric enzyme with small amounts of hexamer | Rattus norvegicus |
H157A | mutant enzyme shows a drastic loss of epimerase activity, loss of epimerase activity can largely by attributed to incorrect protein folding | Rattus norvegicus |
H45A | mutant enzyme shows a drastic loss of epimerase activity | Rattus norvegicus |
R420M | enzyme with mutation in the putative kinase active site shows drastic loss in their kinase activity but retains their epimerase activity | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CMP-N-acetylneuraminic acid | feedback inhibitor | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
6 * 75000, SDS-PAGE | Rattus norvegicus |
450000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | key enzyme of N-acetylneuraminic acid biosynthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | bifunctional enzyme UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase | Rattus norvegicus | ? | - |
? | |
additional information | key enzyme of N-acetylneuraminic acid biosynthesis | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 75000, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
More | bifunctional enzyme UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase | Rattus norvegicus |