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Literature summary for 5.1.3.11 extracted from
- Characterisation of a novel cellobiose 2-epimerase from thermophilic Caldicellulosiruptor obsidiansis for lactulose production (2017), J. Sci. Food. Agric., 97, 3095-3105 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | the recombinant enzyme shows both epimerisation and isomerisation activities against lactose, making it an alternative promising biocatalyst candidate for lactulose production | Caldicellulosiruptor obsidiansis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene COB47_2182, sequence comparisons and phylogenetic tree, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Caldicellulosiruptor obsidiansis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Caldicellulosiruptor obsidiansis | |
| 164.5 | - |
cellobiose | recombinant His6-tagged enzyme, pH 7.5, 70°C | Caldicellulosiruptor obsidiansis |  |
| 301.7 | - |
lactose | recombinant His6-tagged enzyme, pH 7.5, 70°C | Caldicellulosiruptor obsidiansis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
recombinant His6-tagged enzyme, gel filtration | Caldicellulosiruptor obsidiansis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellobiose | Caldicellulosiruptor obsidiansis | - |
4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | Caldicellulosiruptor obsidiansis ATCC BAA-2073 / strain OB47 | - |
4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| lactose | Caldicellulosiruptor obsidiansis | when lactose is used as substrate, epilactose is rapidly produced in a short period, and afterwards both epilactose and lactose are steadily isomerised to lactulose, with a final ratio of 35:11:54 for lactose:epilactose:lactulose | lactulose | - |
r | |
| lactose | Caldicellulosiruptor obsidiansis ATCC BAA-2073 / strain OB47 | when lactose is used as substrate, epilactose is rapidly produced in a short period, and afterwards both epilactose and lactose are steadily isomerised to lactulose, with a final ratio of 35:11:54 for lactose:epilactose:lactulose | lactulose | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caldicellulosiruptor obsidiansis | D9TH31 | - |
- |
| Caldicellulosiruptor obsidiansis ATCC BAA-2073 / strain OB47 | D9TH31 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis, to homogeneity | Caldicellulosiruptor obsidiansis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 93.6 | - |
purified recombinant His6-tagged enzyme, substrate lactose, pH 7.5, 70°C | Caldicellulosiruptor obsidiansis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellobiose | - |
Caldicellulosiruptor obsidiansis | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | preferred substrate | Caldicellulosiruptor obsidiansis | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | - |
Caldicellulosiruptor obsidiansis ATCC BAA-2073 / strain OB47 | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| cellobiose | preferred substrate | Caldicellulosiruptor obsidiansis ATCC BAA-2073 / strain OB47 | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
| lactose | when lactose is used as substrate, epilactose is rapidly produced in a short period, and afterwards both epilactose and lactose are steadily isomerised to lactulose, with a final ratio of 35:11:54 for lactose:epilactose:lactulose | Caldicellulosiruptor obsidiansis | lactulose | - |
r | |
| lactose | when lactose is used as substrate, epilactose is rapidly produced in a short period, and afterwards both epilactose and lactose are steadily isomerised to lactulose, with a final ratio of 35:11:54 for lactose:epilactose:lactulose | Caldicellulosiruptor obsidiansis ATCC BAA-2073 / strain OB47 | lactulose | - |
r | |
| additional information | the recombinant enzyme shows both epimerisation and isomerisation activities against lactose | Caldicellulosiruptor obsidiansis | ? | - |
? | |
| additional information | the recombinant enzyme shows both epimerisation and isomerisation activities against lactose | Caldicellulosiruptor obsidiansis ATCC BAA-2073 / strain OB47 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 46300, about, sequence calculation, 1 * 43000, recombinant His6-tagged enzyme, SDS-PAGE | Caldicellulosiruptor obsidiansis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Caob-CE | - |
Caldicellulosiruptor obsidiansis |
| cellobiose 2-epimerase | - |
Caldicellulosiruptor obsidiansis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Caldicellulosiruptor obsidiansis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
half-life is 77 h | Caldicellulosiruptor obsidiansis |
| 65 | - |
half-life is 57 h | Caldicellulosiruptor obsidiansis |
| 70 | - |
half-life is 23 h | Caldicellulosiruptor obsidiansis |
| 75 | - |
half-life is 8.1 h | Caldicellulosiruptor obsidiansis |
| 80 | - |
half-life is 2.8 h | Caldicellulosiruptor obsidiansis |
| 85 | - |
half-life is 0.6 h | Caldicellulosiruptor obsidiansis |
| 86.7 | - |
the enzyme displays high thermostability with Tm of 86.7 ?C | Caldicellulosiruptor obsidiansis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 233.4 | - |
lactose | recombinant His6-tagged enzyme, pH 7.5, 70°C | Caldicellulosiruptor obsidiansis | |
| 233.7 | - |
cellobiose | recombinant His6-tagged enzyme, pH 7.5, 70°C | Caldicellulosiruptor obsidiansis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Caldicellulosiruptor obsidiansis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.773 | - |
lactose | recombinant His6-tagged enzyme, pH 7.5, 70°C | Caldicellulosiruptor obsidiansis | |
| 1.421 | - |
cellobiose | recombinant His6-tagged enzyme, pH 7.5, 70°C | Caldicellulosiruptor obsidiansis | |
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