Literature summary for 5.1.3.11 extracted from

Krewinkel, M.; Kaiser, J.; Merz, M.; Rentschler, E.; Kuschel, B.; Hinrichs, J.; Fischer, L.
Novel cellobiose 2-epimerases for the production of epilactose from milk ultrafiltrate containing lactose (2015), J. Dairy Sci., 98, 3665-3678 .

Search for more literature for 5.1.3.11

Application

Application	Comment	Organism
nutrition	cellobiose 2-epimerases can be used for the production of epilactose from milk ultrafiltrate containing lactose	Cellulosilyticum lentocellum
nutrition	cellobiose 2-epimerases can be used for the production of epilactose from milk ultrafiltrate containing lactose	Dysgonomonas gadei

Cloned(Commentary)

Cloned (Comment)	Organism
DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli	Cellulosilyticum lentocellum
DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli	Dysgonomonas gadei

Protein Variants

Protein Variants	Comment	Organism
additional information	enzyme production evaluation for large-sclae production, parameters, overview	Cellulosilyticum lentocellum
additional information	enzyme production evaluation for large-sclae production, parameters, overview	Dysgonomonas gadei

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
cellobiose	Cellulosilyticum lentocellum	-	4-O-beta-D-glucopyranosyl-D-mannose	-	r
cellobiose	Dysgonomonas gadei	-	4-O-beta-D-glucopyranosyl-D-mannose	-	r
cellobiose	Cellulosilyticum lentocellum ATCC 49066 / DSM 5427 / NCIMB 11756 / RHM5	-	4-O-beta-D-glucopyranosyl-D-mannose	-	r
cellobiose	Dysgonomonas gadei ATCC BAA-286	-	4-O-beta-D-glucopyranosyl-D-mannose	-	r
lactose	Cellulosilyticum lentocellum	-	epilactose	-	r
lactose	Dysgonomonas gadei	-	epilactose	-	r
lactose	Cellulosilyticum lentocellum ATCC 49066 / DSM 5427 / NCIMB 11756 / RHM5	-	epilactose	-	r
lactose	Dysgonomonas gadei ATCC BAA-286	-	epilactose	-	r

Organism

Organism	UniProt	Comment	Textmining
Cellulosilyticum lentocellum	F2JR71	-	-
Cellulosilyticum lentocellum ATCC 49066 / DSM 5427 / NCIMB 11756 / RHM5	F2JR71	-	-
Dysgonomonas gadei	F5ITJ6	-	-
Dysgonomonas gadei ATCC BAA-286	F5ITJ6	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant enzyme from Escherichia coli	Cellulosilyticum lentocellum
recombinant enzyme from Escherichia coli	Dysgonomonas gadei

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
cellobiose	-	Cellulosilyticum lentocellum	4-O-beta-D-glucopyranosyl-D-mannose	-	r
cellobiose	-	Dysgonomonas gadei	4-O-beta-D-glucopyranosyl-D-mannose	-	r
cellobiose	-	Cellulosilyticum lentocellum ATCC 49066 / DSM 5427 / NCIMB 11756 / RHM5	4-O-beta-D-glucopyranosyl-D-mannose	-	r
cellobiose	-	Dysgonomonas gadei ATCC BAA-286	4-O-beta-D-glucopyranosyl-D-mannose	-	r
lactose	-	Cellulosilyticum lentocellum	epilactose	-	r
lactose	-	Dysgonomonas gadei	epilactose	-	r
lactose	-	Cellulosilyticum lentocellum ATCC 49066 / DSM 5427 / NCIMB 11756 / RHM5	epilactose	-	r
lactose	-	Dysgonomonas gadei ATCC BAA-286	epilactose	-	r

Synonyms

Synonyms	Comment	Organism
cellobiose 2-epimerase	-	Cellulosilyticum lentocellum
cellobiose 2-epimerase	-	Dysgonomonas gadei

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	substrate lactose, recombinant enzyme	Cellulosilyticum lentocellum
40	-	substrate lactose, recombinant enzyme	Dysgonomonas gadei

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	substrate lactose, recombinant enzyme	Dysgonomonas gadei
8	-	substrate lactose, recombinant enzyme	Cellulosilyticum lentocellum

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Release 2023.1 (January 2023)