Cloned (Comment) | Organism |
---|---|
gene epiA, recombinant expression in Escherichia coli | Cellvibrio vulgaris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.03 | - |
4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | pH 8.0, 40°C, recombinant enzyme | Cellvibrio vulgaris | |
24.5 | - |
cellobiose | pH 8.0, 40°C, recombinant enzyme | Cellvibrio vulgaris | |
28.7 | - |
lactose | pH 8.0, 40°C, recombinant enzyme | Cellvibrio vulgaris | |
245 | - |
D-mannose | pH 8.0, 40°C, recombinant enzyme | Cellvibrio vulgaris |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | Cellvibrio vulgaris | - |
4-O-beta-D-mannopyranosyl-D-glucose | - |
r | |
4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | Cellvibrio vulgaris NCIMB8633 | - |
4-O-beta-D-mannopyranosyl-D-glucose | - |
r | |
cellobiose | Cellvibrio vulgaris | - |
4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
cellobiose | Cellvibrio vulgaris NCIMB8633 | - |
4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
lactose | Cellvibrio vulgaris | - |
epilactose | - |
r | |
lactose | Cellvibrio vulgaris NCIMB8633 | - |
epilactose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cellvibrio vulgaris | - |
i.e. Cellvibrio mixtus | - |
Cellvibrio vulgaris NCIMB8633 | - |
i.e. Cellvibrio mixtus | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme EpiA from Escherichia coli | Cellvibrio vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-O-beta-D-glucosyl-D-mannose | - |
Cellvibrio vulgaris | cellobiose | - |
r | |
4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | - |
Cellvibrio vulgaris | 4-O-beta-D-mannopyranosyl-D-glucose | - |
r | |
4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | best substrate | Cellvibrio vulgaris | 4-O-beta-D-mannopyranosyl-D-glucose | - |
r | |
4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | - |
Cellvibrio vulgaris NCIMB8633 | 4-O-beta-D-mannopyranosyl-D-glucose | - |
r | |
cellobiose | - |
Cellvibrio vulgaris | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
cellobiose | - |
Cellvibrio vulgaris NCIMB8633 | 4-O-beta-D-glucopyranosyl-D-mannose | - |
r | |
D-mannose | - |
Cellvibrio vulgaris | D-glucose | - |
r | |
D-mannose | - |
Cellvibrio vulgaris NCIMB8633 | D-glucose | - |
r | |
lactose | - |
Cellvibrio vulgaris | epilactose | - |
r | |
lactose | - |
Cellvibrio vulgaris NCIMB8633 | epilactose | - |
r | |
additional information | epiA has been assigned to encode an epimerase for converting D-mannose to D-glucose, even though the amino acid sequence of EpiA is similar to that of cellobiose 2-epimerases (CEs). Recombinant EpiA catalyzes the epimerization of the 2-OH group of sugar residue at the reducing end of cellobiose, lactose, and beta-(1->4)-mannobiose in a similar manner to other CEs. The reaction efficiency of EpiA for beta-(1->4)-mannobiose is 55000fold higher than it is for D-mannose. Recombinant EpiA does not act on any of the other disaccharides tested, i.e. maltose, isomaltose, sophorose, laminaribiose, gentiobiose, and beta-(1->4)-xylobiose. Recombinant EpiA shows a significant preference for disaccharides over D-mannose. EpiA has high epimerization activity to beta-(1->4)-linked disaccharides, and beta-(1->4)-mannobiose is the best substrate among beta-(1->4)-linked disaccharides | Cellvibrio vulgaris | ? | - |
? | |
additional information | epiA has been assigned to encode an epimerase for converting D-mannose to D-glucose, even though the amino acid sequence of EpiA is similar to that of cellobiose 2-epimerases (CEs). Recombinant EpiA catalyzes the epimerization of the 2-OH group of sugar residue at the reducing end of cellobiose, lactose, and beta-(1->4)-mannobiose in a similar manner to other CEs. The reaction efficiency of EpiA for beta-(1->4)-mannobiose is 55000fold higher than it is for D-mannose. Recombinant EpiA does not act on any of the other disaccharides tested, i.e. maltose, isomaltose, sophorose, laminaribiose, gentiobiose, and beta-(1->4)-xylobiose. Recombinant EpiA shows a significant preference for disaccharides over D-mannose. EpiA has high epimerization activity to beta-(1->4)-linked disaccharides, and beta-(1->4)-mannobiose is the best substrate among beta-(1->4)-linked disaccharides | Cellvibrio vulgaris NCIMB8633 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cellobiose 2-epimerase | - |
Cellvibrio vulgaris |
CvCE | - |
Cellvibrio vulgaris |
EpiA | - |
Cellvibrio vulgaris |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Cellvibrio vulgaris |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 50 | activity range, profile overview | Cellvibrio vulgaris |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
EpiA retains over 90% of its activity at pH values in the range of 5.2-10.0 and temperatures below 40°C following the pH (at 4°C for 24 h) and heat (at pH 8.0 for 30 min) treatment processes, respectively | Cellvibrio vulgaris |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.113 | - |
D-mannose | pH 8.0, 40°C, recombinant enzyme | Cellvibrio vulgaris | |
18.6 | - |
lactose | pH 8.0, 40°C, recombinant enzyme | Cellvibrio vulgaris | |
38.8 | - |
cellobiose | pH 8.0, 40°C, recombinant enzyme | Cellvibrio vulgaris | |
102 | - |
4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | pH 8.0, 40°C, recombinant enzyme | Cellvibrio vulgaris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Cellvibrio vulgaris |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.9 | 9 | narrow activity range, profile overview | Cellvibrio vulgaris |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.2 | 10 | EpiA retains over 90% of its activity at pH values in the range of 5.2-10.0 and temperatures below 40°C following the pH (at 4°C for 24 h) and heat (at pH 8.0 for 30 min) treatment processes, respectively | Cellvibrio vulgaris |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000461 | - |
D-mannose | pH 8.0, 40°C, recombinant enzyme | Cellvibrio vulgaris | |
0.648 | - |
lactose | pH 8.0, 40°C, recombinant enzyme | Cellvibrio vulgaris | |
1.58 | - |
cellobiose | pH 8.0, 40°C, recombinant enzyme | Cellvibrio vulgaris | |
25.3 | - |
4-O-beta-D-mannopyranosyl-beta-D-mannopyranose | pH 8.0, 40°C, recombinant enzyme | Cellvibrio vulgaris |