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Literature summary for 5.1.3.11 extracted from

  • Saburi, W.; Tanaka, Y.; Muto, H.; Inoue, S.; Odaka, R.; Nishimoto, M.; Kitaoka, M.; Mori, H.
    Functional reassignment of Cellvibrio vulgaris EpiA to cellobiose 2-epimerase and an evaluation of the biochemical functions of the 4-O-beta-D-mannosyl-D-glucose phosphorylase-like protein, UnkA (2015), Biosci. Biotechnol. Biochem., 79, 969-977 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene epiA, recombinant expression in Escherichia coli Cellvibrio vulgaris

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.03
-
4-O-beta-D-mannopyranosyl-beta-D-mannopyranose pH 8.0, 40°C, recombinant enzyme Cellvibrio vulgaris
24.5
-
cellobiose pH 8.0, 40°C, recombinant enzyme Cellvibrio vulgaris
28.7
-
lactose pH 8.0, 40°C, recombinant enzyme Cellvibrio vulgaris
245
-
D-mannose pH 8.0, 40°C, recombinant enzyme Cellvibrio vulgaris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-O-beta-D-mannopyranosyl-beta-D-mannopyranose Cellvibrio vulgaris
-
4-O-beta-D-mannopyranosyl-D-glucose
-
r
4-O-beta-D-mannopyranosyl-beta-D-mannopyranose Cellvibrio vulgaris NCIMB8633
-
4-O-beta-D-mannopyranosyl-D-glucose
-
r
cellobiose Cellvibrio vulgaris
-
4-O-beta-D-glucopyranosyl-D-mannose
-
r
cellobiose Cellvibrio vulgaris NCIMB8633
-
4-O-beta-D-glucopyranosyl-D-mannose
-
r
lactose Cellvibrio vulgaris
-
epilactose
-
r
lactose Cellvibrio vulgaris NCIMB8633
-
epilactose
-
r

Organism

Organism UniProt Comment Textmining
Cellvibrio vulgaris
-
i.e. Cellvibrio mixtus
-
Cellvibrio vulgaris NCIMB8633
-
i.e. Cellvibrio mixtus
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme EpiA from Escherichia coli Cellvibrio vulgaris

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-O-beta-D-glucosyl-D-mannose
-
Cellvibrio vulgaris cellobiose
-
r
4-O-beta-D-mannopyranosyl-beta-D-mannopyranose
-
Cellvibrio vulgaris 4-O-beta-D-mannopyranosyl-D-glucose
-
r
4-O-beta-D-mannopyranosyl-beta-D-mannopyranose best substrate Cellvibrio vulgaris 4-O-beta-D-mannopyranosyl-D-glucose
-
r
4-O-beta-D-mannopyranosyl-beta-D-mannopyranose
-
Cellvibrio vulgaris NCIMB8633 4-O-beta-D-mannopyranosyl-D-glucose
-
r
cellobiose
-
Cellvibrio vulgaris 4-O-beta-D-glucopyranosyl-D-mannose
-
r
cellobiose
-
Cellvibrio vulgaris NCIMB8633 4-O-beta-D-glucopyranosyl-D-mannose
-
r
D-mannose
-
Cellvibrio vulgaris D-glucose
-
r
D-mannose
-
Cellvibrio vulgaris NCIMB8633 D-glucose
-
r
lactose
-
Cellvibrio vulgaris epilactose
-
r
lactose
-
Cellvibrio vulgaris NCIMB8633 epilactose
-
r
additional information epiA has been assigned to encode an epimerase for converting D-mannose to D-glucose, even though the amino acid sequence of EpiA is similar to that of cellobiose 2-epimerases (CEs). Recombinant EpiA catalyzes the epimerization of the 2-OH group of sugar residue at the reducing end of cellobiose, lactose, and beta-(1->4)-mannobiose in a similar manner to other CEs. The reaction efficiency of EpiA for beta-(1->4)-mannobiose is 55000fold higher than it is for D-mannose. Recombinant EpiA does not act on any of the other disaccharides tested, i.e. maltose, isomaltose, sophorose, laminaribiose, gentiobiose, and beta-(1->4)-xylobiose. Recombinant EpiA shows a significant preference for disaccharides over D-mannose. EpiA has high epimerization activity to beta-(1->4)-linked disaccharides, and beta-(1->4)-mannobiose is the best substrate among beta-(1->4)-linked disaccharides Cellvibrio vulgaris ?
-
?
additional information epiA has been assigned to encode an epimerase for converting D-mannose to D-glucose, even though the amino acid sequence of EpiA is similar to that of cellobiose 2-epimerases (CEs). Recombinant EpiA catalyzes the epimerization of the 2-OH group of sugar residue at the reducing end of cellobiose, lactose, and beta-(1->4)-mannobiose in a similar manner to other CEs. The reaction efficiency of EpiA for beta-(1->4)-mannobiose is 55000fold higher than it is for D-mannose. Recombinant EpiA does not act on any of the other disaccharides tested, i.e. maltose, isomaltose, sophorose, laminaribiose, gentiobiose, and beta-(1->4)-xylobiose. Recombinant EpiA shows a significant preference for disaccharides over D-mannose. EpiA has high epimerization activity to beta-(1->4)-linked disaccharides, and beta-(1->4)-mannobiose is the best substrate among beta-(1->4)-linked disaccharides Cellvibrio vulgaris NCIMB8633 ?
-
?

Synonyms

Synonyms Comment Organism
cellobiose 2-epimerase
-
Cellvibrio vulgaris
CvCE
-
Cellvibrio vulgaris
EpiA
-
Cellvibrio vulgaris

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Cellvibrio vulgaris

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 50 activity range, profile overview Cellvibrio vulgaris

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
EpiA retains over 90% of its activity at pH values in the range of 5.2-10.0 and temperatures below 40°C following the pH (at 4°C for 24 h) and heat (at pH 8.0 for 30 min) treatment processes, respectively Cellvibrio vulgaris

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.113
-
D-mannose pH 8.0, 40°C, recombinant enzyme Cellvibrio vulgaris
18.6
-
lactose pH 8.0, 40°C, recombinant enzyme Cellvibrio vulgaris
38.8
-
cellobiose pH 8.0, 40°C, recombinant enzyme Cellvibrio vulgaris
102
-
4-O-beta-D-mannopyranosyl-beta-D-mannopyranose pH 8.0, 40°C, recombinant enzyme Cellvibrio vulgaris

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Cellvibrio vulgaris

pH Range

pH Minimum pH Maximum Comment Organism
7.9 9 narrow activity range, profile overview Cellvibrio vulgaris

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.2 10 EpiA retains over 90% of its activity at pH values in the range of 5.2-10.0 and temperatures below 40°C following the pH (at 4°C for 24 h) and heat (at pH 8.0 for 30 min) treatment processes, respectively Cellvibrio vulgaris

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.000461
-
D-mannose pH 8.0, 40°C, recombinant enzyme Cellvibrio vulgaris
0.648
-
lactose pH 8.0, 40°C, recombinant enzyme Cellvibrio vulgaris
1.58
-
cellobiose pH 8.0, 40°C, recombinant enzyme Cellvibrio vulgaris
25.3
-
4-O-beta-D-mannopyranosyl-beta-D-mannopyranose pH 8.0, 40°C, recombinant enzyme Cellvibrio vulgaris