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Literature summary for 5.1.3.11 extracted from

  • Fujiwara, T.; Saburi, W.; Inoue, S.; Mori, H.; Matsui, H.; Tanaka, I.; Yao, M.
    Crystal structure of Ruminococcus albus cellobiose 2-epimerase: structural insights into epimerization of unmodified sugar (2013), FEBS Lett., 587, 840-846.
    View publication on PubMed
Search for more literature for 5.1.3.11

Application

Application Comment Organism
nutrition cellobiose 2-epimerase is an attractive enzyme to produce epilactose (4-O-beta-Dgalactosyl-D-mannose) from lactose. Epilactose is a non-digestible oligosaccharide, and enhances proliferation of bifidobacilli and lactobacilli in the human gut Ruminococcus albus

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Ruminococcus albus

Protein Variants

Protein Variants Comment Organism
H184A site-directed mutagenesis Ruminococcus albus
H184N site-directed mutagenesis Ruminococcus albus
W369A site-directed mutagenesis Ruminococcus albus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Ruminococcus albus cellobiose 2-epimerase catalyzes interconversion between D-glucose and D-mannose residues at the reducing end of beta-1,4-linked oligosaccharides ?
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 ?

Organism

Organism UniProt Comment Textmining
Ruminococcus albus
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Reaction

Reaction Comment Organism Reaction ID
cellobiose = 4-O-beta-D-glucopyranosyl-D-mannose the enzyme's catalytic mechanism involvies three histidine residues, His374 and His243 act as a general acid/base catalyst, and His184 is essential for the enzyme activity, overview Ruminococcus albus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information cellobiose 2-epimerase catalyzes interconversion between D-glucose and D-mannose residues at the reducing end of beta-1,4-linked oligosaccharides Ruminococcus albus ?
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 ?

Subunits

Subunits Comment Organism
More enzyme structure modeling with bound mannose, three-dimensional structure, overview Ruminococcus albus

Synonyms

Synonyms Comment Organism
cellobiose 2-epimerase
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 Ruminococcus albus

General Information

General Information Comment Organism
evolution the structure of RaCE shows strong similarity to those of N-acetyl-D-glucosamine 2-epimerase and aldose-ketose isomerase YihS with a high degree of conservation of residues around the catalytic center, although sequence identity between them is low Ruminococcus albus