Cloned (Comment) | Organism |
---|---|
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Caldicellulosiruptor saccharolyticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is not inhibited by EDTA | Caldicellulosiruptor saccharolyticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is not activated by monovalent or divalent cations | Caldicellulosiruptor saccharolyticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
x * 47000, recombinant His-tagged enzyme, SDS-PAGE | Caldicellulosiruptor saccharolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caldicellulosiruptor saccharolyticus | A4XGA6 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme 10fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Caldicellulosiruptor saccharolyticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
30 | - |
purified recombinnat His-tagged enzyme, substrate lactose, pH 7.5, 80°C | Caldicellulosiruptor saccharolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lactose | cellobiose 2-epimerase from Caldicellulosiruptor saccharolyticus can be used in the presence of borate for the production of lactulose from lactose, method development, overview | Caldicellulosiruptor saccharolyticus | lactulose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 47000, recombinant His-tagged enzyme, SDS-PAGE | Caldicellulosiruptor saccharolyticus |
Synonyms | Comment | Organism |
---|---|---|
cellobiose 2-epimerase | - |
Caldicellulosiruptor saccharolyticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
- |
Caldicellulosiruptor saccharolyticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme displays first-order kinetics for thermal inactivation, and the half-lives (inactivation rate constants) at 65°C, 70°C, 75°C, and 80°C are 74 h, 34 h, 16h , and 5 h, respectively | Caldicellulosiruptor saccharolyticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Caldicellulosiruptor saccharolyticus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the activity of the cellobiose 2-epimerase is not affected by the addition of the cofactor ATP, FAD, FMN, NAD+, NADP+, NADH, or NADPH | Caldicellulosiruptor saccharolyticus |