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Literature summary for 5.1.3.11 extracted from

  • Ito, S.; Hamada, S.; Yamaguchi, K.; Umene, S.; Ito, H.; Matsui, H.; Ozawa, T.; Taguchi, H.; Watanabe, J.; Wasaki, J.; Ito, S.
    Cloning and sequencing of the cellobiose 2-epimerase gene from an obligatory anaerobe, Ruminococcus albus (2007), Biochem. Biophys. Res. Commun., 360, 640-645.
    View publication on PubMed
Search for more literature for 5.1.3.11

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Ruminococcus albus

Protein Variants

Protein Variants Comment Organism
additional information sequence analysis shows that the gene shares a very low level of homology with N-acetyl-D-glucosamine 2-epimerases (EC 5.1.3.8), but no significant homology to any other epimerases reported to date Ruminococcus albus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43100
-
 SDS-PAGE Ruminococcus albus

Organism

Organism UniProt Comment Textmining
Ruminococcus albus P0DKY4
-
-

Purification (Commentary)

Purification (Comment) Organism
protein is purified by loading the extract directly onto coupled columns (15 x 150 mm each) of CM Sepharose Fast Flow and DEAE Sepharose CL-6B Ruminococcus albus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3
-
 the specific activity toward cellobiose is 3–5 micromol/min/mg at a pH optimum 7.8 in 100 mM glycylglycine buffer Ruminococcus albus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cellobiose the enzyme is found to 2-epimerize the reducing terminal glucose moieties of cellotriose and cellotetraose in addition to cellobiose Ruminococcus albus 4-O-beta-D-glucopyranosyl-D-mannose
-
 ?
cellotetraose the enzyme is found to 2-epimerize the reducing terminal glucose moieties of cellotriose and cellotetraose in addition to cellobiose Ruminococcus albus 4-O-beta-D-glucopyranosyl-(1-4)-O-beta-D-glucopyranosyl-(1-4)-O-beta-D-glucopyranosyl-(1-4)-D-mannose
-
 ?
cellotriose the enzyme is found to 2-epimerize the reducing terminal glucose moieties of cellotriose and cellotetraose in addition to cellobiose Ruminococcus albus 4-O-beta-D-glucopyranosyl-(1-4)-O-beta-D-glucopyranosyl-(1-4)-D-mannose
-
 ?

Synonyms

Synonyms Comment Organism
CE-NE1
-
 Ruminococcus albus
cellobiose 2-epimerase
-
 Ruminococcus albus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Ruminococcus albus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
 Ruminococcus albus