Literature summary for 5.1.2.1 extracted from

Rankin, J.A.; Mauban, R.C.; Fellner, M.; Desguin, B.; McCracken, J.; Hu, J.; Varganov, S.A.; Hausinger, R.P.
Lactate racemase nickel-pincer cofactor operates by a proton-coupled hydride transfer mechanism (2018), Biochemistry, 57, 3244-3251 .

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Organism

Organism	UniProt	Comment	Textmining
Lactiplantibacillus plantarum	F9USS9	-	-
Lactiplantibacillus plantarum ATCC BAA-793 / NCIMB 8826 / WCFS1	F9USS9	-	-

Reaction

Reaction	Comment	Organism	Reaction ID
(S)-Lactate = (R)-lactate	the enzyme does not follow a proton-coupled electron transfer mechanism, but a proton-coupled hydride transfer (PCHT) mechanism. The nickel-pincer cofactor facilitates a proton-coupled hydride transfer (PCHT) mechanism during LarA-catalyzed lactate racemization	Lactiplantibacillus plantarum	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(S)-lactate	-	Lactiplantibacillus plantarum	(R)-lactate	-	r
(S)-lactate	-	Lactiplantibacillus plantarum ATCC BAA-793 / NCIMB 8826 / WCFS1	(R)-lactate	-	r

Synonyms

Synonyms	Comment	Organism
LARa	-	Lactiplantibacillus plantarum

Cofactor

Cofactor	Comment	Organism	Structure
additional information	the enzyme contains an organometallic cofactor with nickel coordinated to a covalently tethered pincer ligand, pyridinium-3-thioamide-5-thiocarboxylic acid mononucleotide. The nickel-pincer cofactor facilitates a proton-coupled hydride transfer (PCHT) mechanism during LarA-catalyzed lactate racemization	Lactiplantibacillus plantarum

General Information

General Information	Comment	Organism
additional information	electron paramagnetic resonance spectroscopy of LarA in the absence or presence of substrate revealing a +2 metal oxidation state and inconsistent with a previously proposed proton-coupled electron transfer mechanism. Computational modeling supports hydride transfer to the cofactor at the C4 position or to the nickel atom, but with formation of a nickel-hydride species requiring dissociation of the His200 metal ligand	Lactiplantibacillus plantarum

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Release 2023.1 (January 2023)